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Literature summary for 1.4.3.22 extracted from

  • Rosini, E.; Nossa, S.; Valentino, M.; DArrigo, P.; Marinesco, S.; Pollegioni, L.
    Expression of rat diamine oxidase in Escherichia coli (2012), J. Mol. Catal. B, 82, 115-120.
No PubMed abstract available
Search for more literature for 1.4.3.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami2(DE3) cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
histamine substrate inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 histamine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus
0.45
-
 tyramine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus
0.64
-
 benzylamine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ Cu2+ ions stimulate the activation/maturation of the enzyme. The highest activity is observed at more than 5fold molar ratio between Cu2+ and the enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histamine + H2O + O2 Rattus norvegicus preferred substrate (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + O2
-
 Rattus norvegicus benzaldehyde + NH3 + H2O2
-
 ?
histamine + H2O + O2 preferred substrate Rattus norvegicus (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
phenylethylamine + H2O + O2
-
 Rattus norvegicus ?
-
 ?
tyramine + H2O + O2
-
 Rattus norvegicus (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
DAO
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
30
-
 histamine pH and temperature not specified in the publication Rattus norvegicus