Literature summary for 1.4.3.22 extracted from

Mukherjee, A.; Smirnov, V.V.; Lanci, M.P.; Brown, D.E.; Shepard, E.M.; Dooley, D.M.; Roth, J.P.
Inner-sphere mechanism for molecular oxygen reduction catalyzed by copper amine oxidases (2008), J. Am. Chem. Soc., 130, 9459-9473.

Search for more literature for 1.4.3.22

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00037	-	O2	reaction performed with benzylamine. KM value is too low to be measurable. Therefore, it is calculated from KCAT_KM with putrescine	Pisum sativum
0.0446	-	O2	reaction performed with putrescine	Pisum sativum
0.14	-	putrescine	-	Pisum sativum
0.322	-	benzylamine	-	Pisum sativum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	enzyme binds Cu. It is shown that PSAO exists predominantly in the Cu(I), topaquinone semiquinone state	Pisum sativum

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	Q42432	-	-

Purification (Commentary)

Purification (Comment)	Organism
isolated in 98% purity	Pisum sativum

Reaction

Reaction	Comment	Organism	Reaction ID
histamine + H2O + O2 = (imidazol-4-yl)acetaldehyde + NH3 + H2O2	a mechanistic proposal for O2 reduction is advanced on the basis of thermodynamic considerations together with kinetic studies, the identification of steady-state intermediates, and the analysis of competitive oxygen kinetic isotope effects. The 18O kinetic isotope effect: 1.0136 at pH 7.2 is independent of temperature from 5°C to 47°C and insignificantly changed to 1.0122 upon raising the pH to 9, thus indicating the absence of kinetic complexity	Pisum sativum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzylamine + H2O + O2	-	Pisum sativum	benzaldehyde + NH3 + H2O2	-	?
putrescine + H2O + O2	-	Pisum sativum	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Pisum sativum

Synonyms

Synonyms	Comment	Organism
Copper amine oxidase	-	Pisum sativum
pea seedling amine oxidase	-	Pisum sativum
PSAO	-	Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Pisum sativum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.75	-	benzylamine	-	Pisum sativum
91.5	-	putrescine	-	Pisum sativum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Pisum sativum

Cofactor

Cofactor	Comment	Organism	Structure
2,4,5-trihydroxyphenylalanine quinone	it is shown that PSAO exists predominantly in the Cu(I), topaquinone semiquinone state	Pisum sativum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2100	-	O2	determined with putrescine	Pisum sativum

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Release 2023.1 (January 2023)