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Literature summary for 1.4.3.22 extracted from

  • Duan, J.; Li, J.; Guo, S.; Kang, Y.
    Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance (2008), J. Plant Physiol., 165, 1620-1635.
    View publication on PubMed
Search for more literature for 1.4.3.22

Activating Compound

Activating Compound Comment Organism Structure
NaCl in comparison to cultivar Jinchun no. 2, cultivar Changchun mici shows an increase in diamine oxidase activity as well as free spermidine and spermine, soluble conjugated and insoluble bound putrescine, spermidine and spermine contents in the roots during exposure to salinity (50 mM NaCl). Cucumis sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cucumis sativus polyamine degradation via DAO produces DELTA1-pyrroline, DELTA1-pyrroline can be catabolized to gamma-aminobutyric acid, which is converted via transamination and oxidation to succinate and successively introduced in the Krebs cycle. ?
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 ?

Organism

Organism UniProt Comment Textmining
Cucumis sativus
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 cultivar Changchun mici and Jinchun no. 2
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Source Tissue

Source Tissue Comment Organism Textmining
root
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 Cucumis sativus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 DAO activity is determined by measuring of H2O2 gneration, a product of the polyamine oxidation. Samples are homogenized in 0.1 M potassium phosphate buffer, pH 6.5. Homogenate is centrifuged and the supernatant is used for assay. Reaction mixture contains 2.5 ml of 0.1 M potassium phosphate, pH 6.5, 0.2 ml 4-aminoantipyrine/N,N-dimethylaniline reaction solutions, 0.1 ml of horseradish peroxidase (250 U/ml) and 0.2 ml of the enzyme extract. The reaction is initiated by the addition of 15 microL of putrescine. Salinity stress (50 mM NaCl) causes a significant increase in DAO activity in cultivar Changchun mici roots but only a slight increase in cultivar Jinchun no. 2 roots. When salinity stress is combined with exogenous spermidine treatment, DAO activity increases in cultivar Jinchun no. 2, but only slightly in cv. Changchun mici. Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polyamine degradation via DAO produces DELTA1-pyrroline, DELTA1-pyrroline can be catabolized to gamma-aminobutyric acid, which is converted via transamination and oxidation to succinate and successively introduced in the Krebs cycle. Cucumis sativus ?
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 ?

Synonyms

Synonyms Comment Organism
DAO
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 Cucumis sativus
Diamine oxidase
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 Cucumis sativus