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Literature summary for 1.4.3.22 extracted from

  • Kang, J.H.; Cho, Y.D.
    Purification and properties of diamine oxidase from soybean (Glycine max) (1989), Hanguk Saenghwahakhoe Chi, 22, 361-366.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
spermidine
-
Glycine max
0.065
-
spermine
-
Glycine max
0.53
-
putrescine
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Glycine max
145000
-
gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
axis
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
19.6 units/mg, 1 unit is defined as the change in absorbance of 0.1 at 470 nm/min Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-diaminobutane + H2O + O2
-
Glycine max 4-aminobutanal + NH3 + H2O2
-
?
RCH2NH2 + H2O + O2
-
Glycine max RCHO + NH3 + H2O2
-
?
spermidine + H2O + O2
-
Glycine max ?
-
?
spermine + H2O + O2
-
Glycine max ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
15 min, stable Glycine max
80
-
15 min, complete loss of activity Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5 and pH 10: 50% activity Glycine max