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Literature summary for 1.4.3.21 extracted from

  • McGrath, A.P.; Mithieux, S.M.; Collyer, C.A.; Bakhuis, J.G.; van den Berg, M.; Sein, A.; Heinz, A.; Schmelzer, C.; Weiss, A.S.; Guss, J.M.
    Structure and activity of Aspergillus nidulans copper amine oxidase (2011), Biochemistry, 50, 5718-5730.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2M (NH4)2SO4 and 0.1 M sodium acetate (pH 4.4) with 25% (w/v) PEG 2000 MME Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ contains copper Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-casein + H2O + O2 Aspergillus nidulans the enzyme oxidizes the lysine residues in alpha-casein protein ?
-
?
RCH2NH2 + H2O + O2 Aspergillus nidulans
-
RCHO + NH3 + H2O2
-
?
tropoelastin + H2O + O2 Aspergillus nidulans the enzyme oxidizes the lysine residues in tropoelastin protein ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O + O2 the enzyme oxidizes the lysine residues in alpha-casein protein Aspergillus nidulans ?
-
?
RCH2NH2 + H2O + O2
-
Aspergillus nidulans RCHO + NH3 + H2O2
-
?
tropoelastin + H2O + O2 the enzyme oxidizes the lysine residues in tropoelastin protein Aspergillus nidulans ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Aspergillus nidulans

Synonyms

Synonyms Comment Organism
Copper amine oxidase
-
Aspergillus nidulans
EC 1.4.3.6 formerly Aspergillus nidulans
quinone- and copper-containing amine oxidase
-
Aspergillus nidulans

Cofactor

Cofactor Comment Organism Structure
2,4,5-trihydroxyphenylalanine quinone
-
Aspergillus nidulans