Literature summary for 1.4.3.21 extracted from

Langley, D.B.; Trambaiolo, D.M.; Duff, A.P.; Dooley, D.M.; Freeman, H.C.; Guss, J.M.
Complexes of the copper-containing amine oxidase from Arthrobacter globiformis with the inhibitors benzylhydrazine and tranylcypromine (2008), Acta Crystallogr. Sect. F, 64, 577-583.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminal StrepII-tagged enzyme in complex with inhibitors benzylhydrazine or tranylcypromine, vapour diffusion in hanging drop method, mixing of protein solution containing about 10 mg/ml protein in 50 mM HEPES, pH 7.0, with well solution containing 1.6 M ammonium sulfate and 150 mM sodium citrate pH 7.0. CuSO4, in a twofold molar excess, 2 weeks. The crystals are then transferred to a sitting drop well solution containing 30% v/v glycerol and 2 mM benzylhydrazine dihydrochloride or 0.4 mM tranylcypromine for 30 min, X-ray diffraction structure determination and analysis at 1.65-1.86 A resolution	Arthrobacter globiformis

Crystallization (Comment)

Organism

purified recombinant C-terminal StrepII-tagged enzyme in complex with inhibitors benzylhydrazine or tranylcypromine, vapour diffusion in hanging drop method, mixing of protein solution containing about 10 mg/ml protein in 50 mM HEPES, pH 7.0, with well solution containing 1.6 M ammonium sulfate and 150 mM sodium citrate pH 7.0. CuSO4, in a twofold molar excess, 2 weeks. The crystals are then transferred to a sitting drop well solution containing 30% v/v glycerol and 2 mM benzylhydrazine dihydrochloride or 0.4 mM tranylcypromine for 30 min, X-ray diffraction structure determination and analysis at 1.65-1.86 A resolution

Arthrobacter globiformis

Inhibitors

Inhibitors	Comment	Organism	Structure
benzylhydrazine	forms adducts with the TPQ cofactor, binding structure, overview	Arthrobacter globiformis
tranylcypromine	forms adducts with the TPQ cofactor, also termed (1R,2S)-rel-2-phenylcyclopropanamine, is a mixture of (1R,2S)-2-phenylcyclopropanamine and (1S,2R)-2-phenylcyclopropanamine, binding structure, overview	Arthrobacter globiformis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	bound by three His ligands of the active-site	Arthrobacter globiformis

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter globiformis	P46881	-	-

Synonyms

Synonyms	Comment	Organism
copper-containing amine oxidase	-	Arthrobacter globiformis
quinone-containing copper amine oxidase	-	Arthrobacter globiformis
TPQ-containing CuAO	-	Arthrobacter globiformis

Cofactor

Cofactor	Comment	Organism	Structure
2,4,5-trihydroxyphenylalaninequinone	i.e. TPQ cofactor, the cofactor is spontaneously formed by post-translational modifications of active site amino-acid residues	Arthrobacter globiformis