Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoniazid | 0.2 mM, 42% inhibition of dimeric enzyme, 39% inhibition of tetrameric enzyme | Mycobacterium sp. | |
KCl | 100 mM, 88% inhibition of dimeric and tetrameric enzyme | Mycobacterium sp. | |
additional information | no effect: DTT or EDTA at 1 mM, 1,4-diamino-2-butanone, sodium azide or KCN | Mycobacterium sp. | |
NaCl | 100 mM, 88% inhibition of dimeric and tetrameric enzyme | Mycobacterium sp. | |
o-phenylenediamine | 0.2 mM, 33% inhibition of dimeric enzyme, 26% inhibition of tetrameric enzyme | Mycobacterium sp. | |
Semicarbazide | 0.2 mM, 49% inhibition of dimeric enzyme, 45% inhibition of tetrameric enzyme | Mycobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
benzylamine | dimeric enzyme | Mycobacterium sp. | |
0.005 | - |
benzylamine | tetrameric enzyme | Mycobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the purified enzyme contains 2.39 mol of copper per mol of subunit | Mycobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000, SDS-PAGE | Mycobacterium sp. |
75000 | - |
4 * 75000, SDS-PAGE | Mycobacterium sp. |
150000 | - |
dimer, gel filtration | Mycobacterium sp. |
300000 | - |
tetramer, gel filtration | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
- |
- |
Mycobacterium sp. DSM 3803 / JC1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:benzylamine-grown cell | - |
Mycobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
tetrameric enzyme | Mycobacterium sp. |
6.7 | - |
dimeric enzyme | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | best oxidized substrate | Mycobacterium sp. | benzaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | best oxidized substrate | Mycobacterium sp. DSM 3803 / JC1 | benzaldehyde + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | - |
Mycobacterium sp. | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | - |
Mycobacterium sp. DSM 3803 / JC1 | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Mycobacterium sp. | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Mycobacterium sp. DSM 3803 / JC1 | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, SDS-PAGE | Mycobacterium sp. |
tetramer | 4 * 75000, SDS-PAGE | Mycobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
semicarbazide-sensitive amine oxidase | - |
Mycobacterium sp. |
SSAO | - |
Mycobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
specific activities of dimeric and tetrameric enzyme form in Tris buffer at pH 7.0 is about 6fold lower than those in phosphate buffer at same pH | Mycobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no evidences of topaquinone cofactor involvement | Mycobacterium sp. |