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Literature summary for 1.4.3.2 extracted from

  • Matsui, D.; Im, D.H.; Sugawara, A.; Fukuta, Y.; Fushinobu, S.; Isobe, K.; Asano, Y.
    Mutational and crystallographic analysis of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 Interconversion between oxidase and monooxygenase activities (2014), FEBS Open Bio, 4, 220-228 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.2

Activating Compound

Activating Compound Comment Organism Structure
4-chloromercuribenzoate about 5timer higher activity in the presence of 3 mM 4-chloromercuribenzoate Pseudomonas sp. AIU 813

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. AIU 813

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 8% (w/v) polyethylene glycol 4000 and 0.1 M Na-acetate (pH 4.6) Pseudomonas sp. AIU 813

Protein Variants

Protein Variants Comment Organism
C254I the mutant enzyme shows 5times higher specific activity of oxidase activity than that of the wild type enzyme Pseudomonas sp. AIU 813

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Arg + H2O + O2 Pseudomonas sp. AIU 813
-
 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-Lys + H2O + O2 Pseudomonas sp. AIU 813
-
 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
L-Orn + H2O + O2 Pseudomonas sp. AIU 813
-
 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. AIU 813 W6JQJ6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Pseudomonas sp. AIU 813

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg + H2O + O2
-
 Pseudomonas sp. AIU 813 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
L-Lys + H2O + O2
-
 Pseudomonas sp. AIU 813 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
L-Orn + H2O + O2
-
 Pseudomonas sp. AIU 813 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
L-AAO/MOG
-
 Pseudomonas sp. AIU 813

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas sp. AIU 813