Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (lambdaDE3) | Platichthys stellatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Platichthys stellatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE, 6 m urea ?PAGE, and 2D-PAGE | Platichthys stellatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Platichthys stellatus | the enzyme shows antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Platichthys stellatus | D2KWB6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, native extracellular enzyme from Platichthys stellatus-derived epidermal mucus by ultracentrifugation, hydrophobic interaction chromatography, and gel filtration | Platichthys stellatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal mucus | - |
Platichthys stellatus | - |
gill | - |
Platichthys stellatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus, overview | Platichthys stellatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE, 6 m urea ?PAGE, and 2D-PAGE | Platichthys stellatus |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Platichthys stellatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Platichthys stellatus | isoelectric focusing | - |
5.3 |