Cloned (Comment) | Organism |
---|---|
isozymes MPLAO1, MPLAO2, and MPLAO3, DNA and amino acid sequence determinhation and analysis, phylogenetic analysis and sequence comparisons | Myoxocephalus polyacanthocephalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
L-Lys | 37°C, isozymes MPLAO1 and MPLAO3 | Myoxocephalus polyacanthocephalus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | skin mucus, isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26 | Myoxocephalus polyacanthocephalus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54769 | - |
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation | Myoxocephalus polyacanthocephalus |
56000 | - |
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation | Myoxocephalus polyacanthocephalus |
58000 | - |
x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation | Myoxocephalus polyacanthocephalus |
140000 | - |
isozyme MPLAO3, gel filtration | Myoxocephalus polyacanthocephalus |
145000 | - |
isozyme MPLAO1, gel filtration | Myoxocephalus polyacanthocephalus |
280000 | - |
isozyme MPLAO2, peak 1, gel filtration | Myoxocephalus polyacanthocephalus |
600000 | - |
isozyme MPLAO2, peak 2, gel filtration | Myoxocephalus polyacanthocephalus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys + H2O + O2 | Myoxocephalus polyacanthocephalus | - |
6-amino-2-oxo-hexanoic acid + NH3 + H2O2 | - |
? | |
additional information | Myoxocephalus polyacanthocephalus | MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myoxocephalus polyacanthocephalus | B5U982 | isozymes MPLAO1, MPLAO2, and MPLAO3 | - |
Purification (Comment) | Organism |
---|---|
native isozymes by lectin affinity and anion exchange chromatography, isozyme MPLAO1 and MPLAO3 are purified to homogeneity, while isozyme MPLAO2 is not | Myoxocephalus polyacanthocephalus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin mucus | isozymes MPLAO1, MPLAO2, and MPLAO3 | Myoxocephalus polyacanthocephalus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys + H2O + O2 | - |
Myoxocephalus polyacanthocephalus | 6-amino-2-oxo-hexanoic acid + NH3 + H2O2 | - |
? | |
additional information | MPLAO3 exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, and is most active against Aeromonas salmonicida strain JCM7874, which is attributable to H2O2, because the activity is completely lost in the presence of catalase | Myoxocephalus polyacanthocephalus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 58000, isozyme MPLAO1, SDS-PAGE, x * 56000, isozyme MPLAO3, SDS-PAGE, x * 54769, mature MPLAO3, sequence calculation | Myoxocephalus polyacanthocephalus |
More | isozyme MPLAO3 contains a signal peptide, comprising residues Met1-Ala26, and the mature protein, Val28-Phe520, peptide mapping and sequencing of isozymes MPLAO3 and MPLAO1 | Myoxocephalus polyacanthocephalus |
Synonyms | Comment | Organism |
---|---|---|
LAO | - |
Myoxocephalus polyacanthocephalus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Myoxocephalus polyacanthocephalus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
L-Lys | 37°C, isozyme MPLAO1 | Myoxocephalus polyacanthocephalus | |
4.7 | - |
L-Lys | 37°C, isozyme MPLAO3 | Myoxocephalus polyacanthocephalus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Myoxocephalus polyacanthocephalus | mature MPLAO3, sequence calculation | - |
4.96 |
General Information | Comment | Organism |
---|---|---|
physiological function | the antibacterial LAO isozymes may be involved in the innate immunity of the great sculpin Myoxocephalus polyacanthocephalus skin | Myoxocephalus polyacanthocephalus |