Literature summary for 1.4.3.2 extracted from

Wei, X.L.; Wei, J.F.; Li, T.; Qiao, L.Y.; Liu, Y.L.; Huang, T.; He, S.H.
Purification, characterization and potent lung lesion activity of an L-amino acid oxidase from Agkistrodon blomhoffii ussurensis snake venom (2007), Toxicon, 50, 1126-1139.

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Application

Application	Comment	Organism
medicine	LAO potently induces lesions in lungs and livers. LAO causes severe pneumorrhagia, pulmonary interstitial edema, fusion of pulmonary alveoli, cardiac interstitial edema and bleeding when being intravenously injected into BALB/c mice. Also induces liver cell necrosis and release of cytokines including IL-6, IL-12 and IL-2 from highly purified human peripheral blood monocytes and T cells, respectively. The ability of LAO will contribute to the understanding of the pathogenesis of snakebite wound	Gloydius ussuriensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	L-leucine	-	Gloydius ussuriensis
0.27	-	L-valine	-	Gloydius ussuriensis
0.36	-	L-asparagine	-	Gloydius ussuriensis
0.88	-	L-methionine	-	Gloydius ussuriensis
0.97	-	L-lysine	-	Gloydius ussuriensis
1.57	-	L-tyrosine	-	Gloydius ussuriensis
2.3	-	L-tryptophan	-	Gloydius ussuriensis
2.33	-	L-arginine	-	Gloydius ussuriensis
4.02	-	L-isoleucine	-	Gloydius ussuriensis
4.28	-	L-phenylalanine	-	Gloydius ussuriensis
4.97	-	L-histidine	-	Gloydius ussuriensis
5.12	-	L-alanine	-	Gloydius ussuriensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions	Gloydius ussuriensis
60000	-	2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions	Gloydius ussuriensis
108800	-	gel filtration	Gloydius ussuriensis

Organism

Organism	UniProt	Comment	Textmining
Gloydius ussuriensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration followed by ion-exchange chromatography, 51.3fold	Gloydius ussuriensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Gloydius ussuriensis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.56	-	crude venom	Gloydius ussuriensis
79.92	-	51.3fold purified	Gloydius ussuriensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine + H2O + O2	-	Gloydius ussuriensis	2-oxopropanoic acid + NH3 + H2O2	-	?
L-arginine + H2O + O2	-	Gloydius ussuriensis	2-oxo-5-guanidinovaleric acid + NH3 + H2O2	-	?
L-asparagine + H2O + O2	-	Gloydius ussuriensis	alpha-ketosuccinamic acid + NH3 + H2O2	-	?
L-histidine + H2O + O2	-	Gloydius ussuriensis	3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2	-	?
L-isoleucine + H2O + O2	-	Gloydius ussuriensis	3-methyl-2-oxopentanoic acid + NH3 + H2O2	-	?
L-leucine + H2O + O2	-	Gloydius ussuriensis	4-methyl-2-oxopentanoic acid + NH3 + H2O2	-	?
L-lysine + H2O + O2	-	Gloydius ussuriensis	6-amino-2-oxohexanoic acid + NH3 + H2O2	-	?
L-methionine + H2O + O2	-	Gloydius ussuriensis	4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2	-	?
L-phenylalanine + H2O + O2	-	Gloydius ussuriensis	phenylpyruvate + NH3 + H2O2	-	?
L-tryptophan + H2O + O2	-	Gloydius ussuriensis	3-indole-2-oxopropanoate + NH3 + H2O2	-	?
L-tyrosine + H2O + O2	-	Gloydius ussuriensis	p-hydroxyphenylpyruvate + NH3 + H2O2	-	?
L-valine + H2O + O2	-	Gloydius ussuriensis	2-oxoisovalerate + NH3 + H2O2	-	?
additional information	does not hydrolyze casein and synthetic substrates including N-benzoyl-L-arginine ethylester or N-benzoyl-L-arginine p-nitroanilide	Gloydius ussuriensis	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions	Gloydius ussuriensis

Synonyms

Synonyms	Comment	Organism
LAO	-	Gloydius ussuriensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.15	-	L-histidine	-	Gloydius ussuriensis
5.08	-	L-valine	-	Gloydius ussuriensis
5.26	-	L-asparagine	-	Gloydius ussuriensis
6.33	-	L-isoleucine	-	Gloydius ussuriensis
6.58	-	L-tryptophan	-	Gloydius ussuriensis
8.42	-	L-tyrosine	-	Gloydius ussuriensis
10.51	-	L-lysine	-	Gloydius ussuriensis
22.54	-	L-arginine	-	Gloydius ussuriensis
24.13	-	L-methionine	-	Gloydius ussuriensis
26.89	-	L-alanine	-	Gloydius ussuriensis
48.22	-	L-leucine	-	Gloydius ussuriensis
48.23	-	L-phenylalanine	-	Gloydius ussuriensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.2	-	-	Gloydius ussuriensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.4	9.6	-	Gloydius ussuriensis

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Release 2023.1 (January 2023)