Application | Comment | Organism |
---|---|---|
additional information | the enzyme has antibacterial activity inhibiting the growth of Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria. LAAO dose-dependently inhibits ADP- or collagen-induced platelet aggregation with IC50 of 0.094 micromol and 0.036 micromol, respectively | Naja oxiana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the antibacterial and anti-aggregating activity of LAAO is abolished by catalase | Naja oxiana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
L-phenylalanine | - |
Naja oxiana | |
0.147 | - |
L-tryptophan | - |
Naja oxiana | |
0.75 | - |
L-leucine | - |
Naja oxiana | |
0.885 | - |
L-methionine | - |
Naja oxiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
1 * 57000, SDS-PAGE | Naja oxiana |
110000 | - |
gel filtration | Naja oxiana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Naja oxiana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, ion exchange chromatography, 100fold purified | Naja oxiana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Naja oxiana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
activity against L-lysine | Naja oxiana |
0.38 | - |
activity against L-glutamine | Naja oxiana |
0.5 | - |
activity against L-isoleucine | Naja oxiana |
0.67 | - |
activity against L-tyrosine | Naja oxiana |
0.81 | - |
activity against L-glutaminic acid | Naja oxiana |
1.19 | - |
activity against L-histidine | Naja oxiana |
5.75 | - |
activity against L-arginine | Naja oxiana |
8.75 | - |
activity against L-tryptophan | Naja oxiana |
9.38 | - |
activity against L-phenylalanine | Naja oxiana |
15 | - |
activity against L-leucine | Naja oxiana |
21 | - |
activity against L-methionine | Naja oxiana |
Storage Stability | Organism |
---|---|
4°C, several months | Naja oxiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O + O2 | good substrate | Naja oxiana | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | good substrate | Naja oxiana | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | good substrate | Naja oxiana | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | good substrate | Naja oxiana | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | good substrate | Naja oxiana | 3-indole-2-oxopropanoate + NH3 + H2O2 | - |
? | |
additional information | L-histidine, L-glutaminic acid, L-tyrosine, L-isoleucine, L-glutamine and L-lysine are poor substrates | Naja oxiana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Naja oxiana |
monomer | 1 * 57000, SDS-PAGE | Naja oxiana |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Naja oxiana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-70 | - |
the enzyme is stable at low temperatures (-20°C, -70°C) for one week and completely loses its activity after heating at 70°C for 10 min | Naja oxiana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.18 | - |
L-phenylalanine | - |
Naja oxiana | |
47.98 | - |
L-leucine | - |
Naja oxiana | |
66.26 | - |
L-methionine | - |
Naja oxiana | |
81.04 | - |
L-tryptophan | - |
Naja oxiana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Naja oxiana | isoelectric focusing | - |
8 |