Application | Comment | Organism |
---|---|---|
diagnostics | a rapid and simple glycine analysis method is developed using a stable glycine oxidase mutant (T42 A/C245 S/L301V). Glycine analysis is important in research fields such as physiology and healthcare because the concentration of glycine in human plasma has been reported to change with various disorders | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
L301V | improvement in residual activity after incubation of 60 min at 60°C to 12.9% | Bacillus subtilis |
T42A | improvement in residual activity after incubation of 60 min at 60°C to 18.7% | Bacillus subtilis |
T42A/C245S/L301V | improvement in residual activity after incubation of 60 min at 60°C to 74.0%. The mutant enzyme retains most of its enzymatic activity during storage for over a year at 4°C | Bacillus subtilis |
T42S | improvement in residual activity after incubation of 60 min at 60°C to 31.5% | Bacillus subtilis |
T42S/C245S/L301V | improvement in residual activity after incubation of 60 min at 60°C to 55.7% | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
glycine | pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V | Bacillus subtilis | |
0.99 | - |
glycine | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | Bacillus subtilis | - |
glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | Bacillus subtilis 168 | - |
glyoxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31616 | - |
- |
Bacillus subtilis 168 | O31616 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Bacillus subtilis 168 | glyoxylate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlyOX | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, residual activity of wild-type is almost absent, activity of C245S mutant is 4.4% | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glycine | pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V | Bacillus subtilis | |
1.3 | - |
glycine | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
glycine | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
6.4 | - |
glycine | pH 8.5, 37°C, mutant enzyme T42A/C245S/L301V | Bacillus subtilis |