Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A54E | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
A54R | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
A54R/H244K | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
A54R/H244K/M261R | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
G51H | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
G51Q | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
H244A | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
H244K | the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
H244K/M261R | the variant shows a 5.4fold increase in maximal activity on glycine compared to the wild type enzyme | Bacillus subtilis |
H244N | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
H244Q | the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
H244R | the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M261I | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M261R | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M49I | the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M49L | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M49L/A54R/H244K | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
M49T | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
Y246W | the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
glycine | mutant enzyme H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
0.24 | - |
glycine | mutant enzyme H244R, at pH 8.5 and 25°C | Bacillus subtilis | |
0.3 | - |
glycine | mutant enzyme H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
0.31 | - |
glycine | mutant enzyme M49I, at pH 8.5 and 25°C | Bacillus subtilis | |
0.7 | - |
glycine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
0.7 | - |
sarcosine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
0.9 | - |
glycine | mutant enzyme H244Q, at pH 8.5 and 25°C | Bacillus subtilis | |
1.35 | - |
glycine | mutant enzyme M49L, at pH 8.5 and 25°C | Bacillus subtilis | |
1.47 | - |
glycine | mutant enzyme M49T, at pH 8.5 and 25°C | Bacillus subtilis | |
1.5 | - |
glycine | mutant enzyme H244A, at pH 8.5 and 25°C | Bacillus subtilis | |
1.5 | - |
glycine | mutant enzyme M261I, at pH 8.5 and 25°C | Bacillus subtilis | |
1.9 | - |
glycine | mutant enzyme H244N, at pH 8.5 and 25°C | Bacillus subtilis | |
3.5 | - |
glycine | mutant enzyme M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
10 | - |
glycine | mutant enzyme A54E, at pH 8.5 and 25°C | Bacillus subtilis | |
14.6 | - |
glycine | mutant enzyme G51Q, at pH 8.5 and 25°C | Bacillus subtilis | |
17.2 | - |
glycine | mutant enzyme Y246W, at pH 8.5 and 25°C | Bacillus subtilis | |
18.8 | - |
glycine | mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
28 | - |
glycine | mutant enzyme A54R, at pH 8.5 and 25°C | Bacillus subtilis | |
35.7 | - |
glycine | mutant enzyme G51H, at pH 8.5 and 25°C | Bacillus subtilis | |
40.1 | - |
glycine | mutant enzyme A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
45.9 | - |
glycine | mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31616 | - |
- |
Bacillus subtilis 168 | O31616 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelating column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Bacillus subtilis 168 | glyoxylate + NH3 + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus subtilis 168 | glyoxylate + methylamine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 47000, SDS-PAGE | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
melting temperature | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
glycine | mutant enzyme M49T, at pH 8.5 and 25°C | Bacillus subtilis | |
0.35 | - |
glycine | mutant enzyme A54E, at pH 8.5 and 25°C | Bacillus subtilis | |
0.36 | - |
glycine | mutant enzyme M49I, at pH 8.5 and 25°C | Bacillus subtilis | |
0.43 | - |
glycine | mutant enzyme M49L, at pH 8.5 and 25°C | Bacillus subtilis | |
0.6 | - |
glycine | mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
0.6 | - |
glycine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
0.6 | - |
sarcosine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
0.63 | - |
glycine | mutant enzyme H244A, at pH 8.5 and 25°C | Bacillus subtilis | |
0.64 | - |
glycine | mutant enzyme M261I, at pH 8.5 and 25°C | Bacillus subtilis | |
0.74 | - |
glycine | mutant enzyme G51Q, at pH 8.5 and 25°C | Bacillus subtilis | |
0.77 | - |
glycine | mutant enzyme G51H, at pH 8.5 and 25°C | Bacillus subtilis | |
1 | - |
glycine | mutant enzyme M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
1.2 | - |
glycine | mutant enzyme A54R, at pH 8.5 and 25°C | Bacillus subtilis | |
1.2 | - |
glycine | mutant enzyme H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
1.21 | - |
glycine | mutant enzyme H244Q, at pH 8.5 and 25°C | Bacillus subtilis | |
1.21 | - |
glycine | mutant enzyme Y246W, at pH 8.5 and 25°C | Bacillus subtilis | |
1.3 | - |
glycine | mutant enzyme H244N, at pH 8.5 and 25°C | Bacillus subtilis | |
1.35 | - |
glycine | mutant enzyme H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
1.49 | - |
glycine | mutant enzyme H244R, at pH 8.5 and 25°C | Bacillus subtilis | |
1.61 | - |
glycine | mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
3.2 | - |
glycine | mutant enzyme A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
glycine | mutant enzyme G51H, at pH 8.5 and 25°C | Bacillus subtilis | |
0.03 | - |
glycine | mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
0.03 | - |
glycine | mutant enzyme M49T, at pH 8.5 and 25°C | Bacillus subtilis | |
0.04 | - |
glycine | mutant enzyme A54E, at pH 8.5 and 25°C | Bacillus subtilis | |
0.04 | - |
glycine | mutant enzyme A54R, at pH 8.5 and 25°C | Bacillus subtilis | |
0.04 | - |
glycine | mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
0.05 | - |
glycine | mutant enzyme G51Q, at pH 8.5 and 25°C | Bacillus subtilis | |
0.07 | - |
glycine | mutant enzyme Y246W, at pH 8.5 and 25°C | Bacillus subtilis | |
0.11 | - |
glycine | mutant enzyme A54R/H244K, at pH 8.5 and 25°C | Bacillus subtilis | |
0.3 | - |
glycine | mutant enzyme M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
0.32 | - |
glycine | mutant enzyme M49L, at pH 8.5 and 25°C | Bacillus subtilis | |
0.42 | - |
glycine | mutant enzyme H244A, at pH 8.5 and 25°C | Bacillus subtilis | |
0.43 | - |
glycine | mutant enzyme M261I, at pH 8.5 and 25°C | Bacillus subtilis | |
0.68 | - |
glycine | mutant enzyme H244N, at pH 8.5 and 25°C | Bacillus subtilis | |
0.86 | - |
glycine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
0.86 | - |
sarcosine | wild type enzyme, at pH 8.5 and 25°C | Bacillus subtilis | |
1.17 | - |
glycine | mutant enzyme M49I, at pH 8.5 and 25°C | Bacillus subtilis | |
1.34 | - |
glycine | mutant enzyme H244Q, at pH 8.5 and 25°C | Bacillus subtilis | |
4 | - |
glycine | mutant enzyme H244K/M261R, at pH 8.5 and 25°C | Bacillus subtilis | |
6.22 | - |
glycine | mutant enzyme H244R, at pH 8.5 and 25°C | Bacillus subtilis | |
9.65 | - |
glycine | mutant enzyme H244K, at pH 8.5 and 25°C | Bacillus subtilis |