Cloned (Comment) | Organism |
---|---|
cloning of the glycine oxidase gene from Bacillus subtilis ATCC 6633 into the Rosetta Escherichia coli strain which contains the pRARE plasmid, inserting of the gene into the pET28a expression vector | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
M261H | decreased in Km compared with the wild type | Bacillus subtilis |
M261Y | decreased in Km compared with the wild type | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
glycine | M261Y mutant | Bacillus subtilis | |
0.23 | - |
sarcosine | M261Y mutant | Bacillus subtilis | |
0.29 | - |
glycine-ethyl-ester | M261Y mutant | Bacillus subtilis | |
0.56 | - |
glycine | M261 wild type | Bacillus subtilis | |
0.57 | - |
sarcosine | M261 wild type | Bacillus subtilis | |
0.58 | - |
sarcosine | M261H mutant | Bacillus subtilis | |
0.71 | - |
N-ethyl-glycine | M261Y mutant | Bacillus subtilis | |
0.97 | - |
glycine | M261H mutant | Bacillus subtilis | |
1.57 | - |
glycine-ethyl-ester | M261H mutant | Bacillus subtilis | |
1.78 | - |
glycine-ethyl-ester | M261 wild type | Bacillus subtilis | |
2.06 | - |
D-proline | M261Y mutant | Bacillus subtilis | |
2.07 | - |
N-ethyl-glycine | M261H mutant | Bacillus subtilis | |
2.8 | - |
N-ethyl-glycine | M261 wild type | Bacillus subtilis | |
3.91 | - |
D-proline | M261H mutant | Bacillus subtilis | |
8.9 | - |
D-proline | M261 wild type | Bacillus subtilis | |
121.9 | - |
D-alanine | M261Y mutant | Bacillus subtilis | |
155.6 | - |
D-alanine | M261H mutant | Bacillus subtilis | |
315 | - |
D-alanine | M261 wild type | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
monomer, determined by SDS-PAGE | Bacillus subtilis |
172000 | - |
homotetramer, determined by gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | Bacillus subtilis | - |
pyruvate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | Bacillus subtilis | - |
? | - |
? | |
glycine + H2O + O2 | Bacillus subtilis | - |
glyoxylate + NH3 + H2O2 | - |
? | |
glycine-ethyl-ester + H2O + O2 | Bacillus subtilis | - |
? | - |
? | |
N-ethylglycine + H2O + O2 | Bacillus subtilis | - |
glyoxylate + ethylamine + H2O2 | - |
? | |
sarcosine + H2O + O2 | Bacillus subtilis | - |
glyoxylate + methylamine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ultrafiltation and a HiTrap chelating affinity column | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
crude extract | Bacillus subtilis |
0.21 | - |
purification step ultrafiltration | Bacillus subtilis |
1.2 | - |
purification step HiTrap chelating chromatography | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-80°C, 75 mM sodium diphosphate buffer, pH 8.5, 10% glycerol | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Bacillus subtilis | pyruvate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | - |
Bacillus subtilis | ? | - |
? | |
glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
? | |
glycine-ethyl-ester + H2O + O2 | - |
Bacillus subtilis | ? | - |
? | |
N-ethylglycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + ethylamine + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
glycin oxidase | - |
Bacillus subtilis |
GOX | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus subtilis |