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Literature summary for 1.4.3.16 extracted from

  • Hao, J.; Petriacq, P.; de Bont, L.; Hodges, M.; Gakiere, B.
    Characterization of L-aspartate oxidase from Arabidopsis thaliana (2018), Plant Sci., 271, 133-142 .
    View publication on PubMed
Search for more literature for 1.4.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
NADH inhibits the enzyme at physiological concentrations by competing with the flavin for binding to the apoprotein Arabidopsis thaliana
NADP+ inhibits the enzyme at physiological concentrations by competing with the flavin for binding to the apoprotein Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
 L-aspartate pH 8.0, 20°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 SDS-PAGE Arabidopsis thaliana
127000
-
 native gel electrophoresis Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + H2O + fumarate Arabidopsis thaliana
-
 oxaloacetate + NH3 + succinate
-
 ?
L-aspartate + O2 Arabidopsis thaliana first enzyme of the de novo biosynthetic pathway of NAD+ in plants iminosuccinate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + H2O + fumarate
-
 Arabidopsis thaliana oxaloacetate + NH3 + succinate
-
 ?
L-aspartate + O2
-
 Arabidopsis thaliana iminosuccinate + H2O2
-
 ?
L-aspartate + O2 first enzyme of the de novo biosynthetic pathway of NAD+ in plants Arabidopsis thaliana iminosuccinate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At5g14760
-
 Arabidopsis thaliana
LASPO
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 55% of maximal activity, 65°C: about 40% of maximal activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 L-aspartate pH 8.0, 20°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7 12 pH 7.0: about 55% of maximal activity, pH 12.0: about 80% of maximal activity Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the enzyme catalyzes a key metabolic step for the production of the nucleotide cofactor NAD+ Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.32
-
 L-aspartate pH 8.0, 20°C Arabidopsis thaliana