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Literature summary for 1.4.3.16 extracted from

  • Sakuraba, H.; Yoneda, K.; Asai, I.; Tsuge, H.; Katunuma, N.; Ohshima, T.
    Structure of L-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii (2008), Biochim. Biophys. Acta, 1784, 563-571.
    View publication on PubMed
Search for more literature for 1.4.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) codon plus RIL Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 23% (w/v) polyethylene glycol 8000, 0.2 M MgCl2 and 0.1 M Tris/HCl buffer (pH 8.6) Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 L-aspartate recombinant enzyme, at 37°C Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Sulfurisphaera tokodaii
52000
-
 recombinant enzyme, SDS-PAGE Sulfurisphaera tokodaii
52580
-
 calculated from the amino acid sequence Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Butyl Toyopearl column chromatography Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific activity of the purified enzyme is estimated to be 0.7 units/mg at 37°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + H2O + O2
-
 Sulfurisphaera tokodaii oxaloacetate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, gel filtration Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
LAO
-
 Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 the enzyme retains more than 90% of its activity when heated for 10 min at 80°C, the enzyme retains 70% of its activity after incubation for 1 h at 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 around pH 8.0 Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.7 10.3 when heated at 50 °C for 30 min, the enzyme does not lose activity at pH values from 3.7 to 10.3 Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Sulfurisphaera tokodaii