Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) codon plus RIL | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 23% (w/v) polyethylene glycol 8000, 0.2 M MgCl2 and 0.1 M Tris/HCl buffer (pH 8.6) | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-aspartate | recombinant enzyme, at 37°C | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Sulfurisphaera tokodaii |
52000 | - |
recombinant enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
52580 | - |
calculated from the amino acid sequence | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Butyl Toyopearl column chromatography | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the purified enzyme is estimated to be 0.7 units/mg at 37°C | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + O2 | - |
Sulfurisphaera tokodaii | oxaloacetate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, gel filtration | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
LAO | - |
Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
the enzyme retains more than 90% of its activity when heated for 10 min at 80°C, the enzyme retains 70% of its activity after incubation for 1 h at 80°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
around pH 8.0 | Sulfurisphaera tokodaii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.7 | 10.3 | when heated at 50 °C for 30 min, the enzyme does not lose activity at pH values from 3.7 to 10.3 | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sulfurisphaera tokodaii |