Cloned (Comment) | Organism |
---|---|
expression of mature enzyme and C-terminally deleted enzyme as N-terminally His6-tagged enzymes | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the interaction with elastin and histones for recombinant mature enzyme and C-terminally deleted enzyme form | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE | Homo sapiens |
34000 | - |
x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-L-lysyl-peptide + O2 + H2O | Homo sapiens | key enzyme in formation of lysine cross-links in side collagen and elastin molecules | peptidyl-allysyl-peptide + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation of the proenzyme by cleavage, enzyme contains a BMP-1 cleavage site | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mature enzyme and C-terminally deleted enzyme by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H1 + O2 + H2O | recombinant mature LOX and C-terminally deleted enzyme | Homo sapiens | ? | - |
? | |
histone H2 + O2 + H2O | recombinant mature LOX and C-terminally deleted enzyme | Homo sapiens | ? | - |
? | |
peptidyl-L-lysyl-peptide + O2 + H2O | key enzyme in formation of lysine cross-links in side collagen and elastin molecules | Homo sapiens | peptidyl-allysyl-peptide + NH3 + H2O2 | - |
? | |
peptidyl-L-lysyl-peptide + O2 + H2O | in collagen and elastin fibrills | Homo sapiens | peptidyl-allysyl-peptide + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE | Homo sapiens |
More | domain structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LOX | - |
Homo sapiens |
lysyl oxidase | - |
Homo sapiens |
protein-6-oxidase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains a cross-linked quinone, cross-linking via lysyl- and tyrosyl residues | Homo sapiens |