Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.11 extracted from

  • Arima, J.; Sasaki, C.; Sakaguchi, C.; Mizuno, H.; Tamura, T.; Kashima, A.; Kusakabe, H.; Sugio, S.; Inagaki, K.
    Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6 (2009), FEBS J., 276, 3894-3903.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of mature LGOX are grown at 5°C using the sitting drop vapor diffusion method. The LGOX crystals are formed in the presence of alpha-ketoglutarate. LGOX has a deeply buried active site and two entrances from the surface of the protein into the active site. Comparison of the LGOX structure with that of a structurally homologous model of L-amino acid oxidase (LAAO) from snake venom reveales that LGOX has three regions that are absent from the LAAO structure, one of which is involved in the formation of the entrance. The arrangement of the residues composing the active site differs between LGOX and LAAO, and the active site of LGOX is narrower than that of LAAO Streptomyces sp. X-119-6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
mature LGOX SDS-PAGE and mass spectroscopy Streptomyces sp. X-119-6
75000
-
precursor protein, SDS-PAGE and mass spectroscopy Streptomyces sp. X-119-6

Organism

Organism UniProt Comment Textmining
Streptomyces sp. X-119-6
-
-
-

Subunits

Subunits Comment Organism
hexamer mature enzyme forms a hexameric alpha2beta2gamma2 structure Streptomyces sp. X-119-6

Synonyms

Synonyms Comment Organism
L-glutamate oxidase
-
Streptomyces sp. X-119-6
LGOX
-
Streptomyces sp. X-119-6