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Literature summary for 1.4.3.11 extracted from

  • Upadhyay, S.; Ohgami, N.; Kusakabe, H.; Mizuno, H.; Arima, J.; Tamura, T.; Inagaki, K.; Suzuki, H.
    Performance characterization of recombinant L-glutamate oxidase in a micro GOT/GPT sensing system (2006), Sens. Actuators B Chem., B119, 570-576.
No PubMed abstract available
Search for more literature for 1.4.3.11

Application

Application Comment Organism
medicine recombinant l-GlOx may be used for food analysis or in the monitoring of L-glutamate as a neurotransmitter, can be used to construct micro sensors and microanalysis systems, performs very similarly to the commercially available wild-type l-GlOx, the recombinant l-GlOx is suitable for mass production Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli JM109 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 1 * 40000, 1 * 17000, and 1 * 12000, SDS–PAGE, recombinant enzyme Streptomyces sp.
17000
-
 1 * 40000, 1 * 17000, and 1 * 12000, SDS–PAGE, recombinant enzyme Streptomyces sp.
40000
-
 1 * 40000, 1 * 17000, and 1 * 12000, SDS–PAGE, recombinant enzyme Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + O2 + H2O slight catalytic activity with the micro L-glutamate sensor with recombinant l-GlOx Streptomyces sp. ?
-
 ?
L-aspartate + O2 + H2O slight catalytic activity with the micro L-glutamate sensor with recombinant l-GlOx Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O
-
 Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? 1 * 40000, 1 * 17000, and 1 * 12000, SDS–PAGE, recombinant enzyme Streptomyces sp.

Synonyms

Synonyms Comment Organism
l-GlOx
-
 Streptomyces sp.