Application | Comment | Organism |
---|---|---|
biotechnology | potential of putrescine oxidase for the bioproduction of N-heterocycles from cadaverine. Complete biotransformation of cadaverine is observed in whole cells at physiological conditions | Rhodococcus erythropolis |
synthesis | potential of putrescine oxidase for the bioproduction of N-heterocycles from cadaverine | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition at concentrations as high as 100 mM/l cadaverine | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | B0F9F6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cadaverine + O2 + H2O | - |
Rhodococcus erythropolis | 5-aminopentanal + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Re-PuO | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 75 | 35°C: about 50% of maximal activity, 75°C: about 60% of maximal activity | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 7.5, the enzyme retains its activity after 65 h incubation | Rhodococcus erythropolis |
55 | - |
pH 7.5, the enzyme loses 75% of its activity after 1 h incubation at 55°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 60% of maximal activity, pH 9.5: about 80% of maximal activity | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
the enzyme retains its activity after 65 h incubation at 25°C, but lost 75% of its activity after 1 h incubation at 55°C | Rhodococcus erythropolis |