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Literature summary for 1.4.3.10 extracted from

  • Yang, T.; Kim, Y.; Roy, J.; Kim, Y.
    Combined cross-linked enzyme aggregates of monoamine oxidase and putrescine oxidase as a bifunctional biocatalyst for determination of biogenic amines in foods (2019), Catalysts, 9, 579 .
No PubMed abstract available
Search for more literature for 1.4.3.10

Application

Application Comment Organism
diagnostics in order to determine total biogenic amines in fermented foods, the combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs) are prepared. The combined cross-linked enzyme aggregate of monoamine oxidase and putrescine oxidase is a promising catalyst with the improved stability and the same optimum pH for dual activities in enzymatic determination of biogenic amines in foods Paenarthrobacter aurescens

Inhibitors

Inhibitors Comment Organism Structure
putrescine inhibition potency of the enzyme in the combined cross-linked enzyme aggregate of monoamine oxidase and putrescine oxidase by the substrate is reduced by two-fold in comparison of the mixed free enzymes Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
putrescine + O2 + H2O the catalytic effciency of the combined cross-linked enzyme aggregates of monoamine oxidase and putrescine oxidase for putrescine is reduced by 41% Paenarthrobacter aurescens 4-aminobutanal + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
APUO
-
 Paenarthrobacter aurescens
putrescine oxidase
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 soluble enzyme and aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs) Paenarthrobacter aurescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life: 117 min, soluble enzyme. Half-life: 136 min, combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs) Paenarthrobacter aurescens
65
-
 half-life: 22.3 min, soluble enzyme. Half-life: 46.8 min, combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs) Paenarthrobacter aurescens
70
-
 half-life: 0.89 min, soluble enzyme. Half-life: 4.7 min, combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs) Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 soluble enzyme Paenarthrobacter aurescens