Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rhodococcus erythropolis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 2.0-2.4 M ammonium sulfate, 0.1 M sodium citrate, and 0.1 M MES-HCl pH 6.4 | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
A394C | the mutant enzyme does not contain covalently bound flavin | Rhodococcus erythropolis |
A394C/A396T/Q431G | mutant with strongly reduced catalytic efficiency | Rhodococcus erythropolis |
A394C/V433M | the mutations improve the affinity toward FAD | Rhodococcus erythropolis |
P15I/A394C | the mutations improve the affinity toward FAD | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0134 | - |
putrescine | mutant enzyme P15I/A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
0.0197 | - |
putrescine | mutant enzyme A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
0.021 | - |
putrescine | mutant enzyme A394C/A396T/Q431G, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
0.0454 | - |
putrescine | wild type enzyme, at pH 8.3 and 25°C | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + O2 + H2O | Rhodococcus erythropolis | - |
4-aminobutanal + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | B0F9F6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + O2 + H2O | - |
Rhodococcus erythropolis | 4-aminobutanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
PuO | - |
Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
putrescine | mutant enzyme P15I/A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
0.95 | - |
putrescine | mutant enzyme A394C/A396T/Q431G, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
2.4 | - |
putrescine | mutant enzyme A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
19 | - |
putrescine | wild type enzyme, at pH 8.3 and 25°C | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | ADP competes with FAD, about 50% of wild type monomers isolated are occupied by ADP instead of FAD | Rhodococcus erythropolis | |
FAD | - |
Rhodococcus erythropolis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
putrescine | mutant enzyme A394C/A396T/Q431G, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
59 | - |
putrescine | mutant enzyme P15I/A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
123 | - |
putrescine | mutant enzyme A394C, at pH 8.3 and 25°C | Rhodococcus erythropolis | |
419 | - |
putrescine | wild type enzyme, at pH 8.3 and 25°C | Rhodococcus erythropolis |