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Literature summary for 1.4.3.10 extracted from

  • Yamada, H.
    Putrescine oxidase (Micrococcus rubens) (1971), Methods Enzymol., 17B, 726-730.
No PubMed abstract available
Search for more literature for 1.4.3.10

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
 Kocuria rosea
hydroxylamine
-
 Kocuria rosea
Iproniazid
-
 Kocuria rosea
PCMB
-
 Kocuria rosea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 spermidine
-
 Kocuria rosea
0.23
-
 putrescine
-
 Kocuria rosea

Organism

Organism UniProt Comment Textmining
Kocuria rosea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kocuria rosea

Storage Stability

Storage Stability Organism
-22°C, stable for months Kocuria rosea
5°C, 0.01 M phosphate buffer, pH 6.0-8.0, little loss of activity Kocuria rosea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cadaverine + O2 + H2O
-
 Kocuria rosea 5-aminopentanal + NH3 + H2O2
-
 ?
putrescine + O2 + H2O
-
 Kocuria rosea 4-aminobutanal + NH3 + H2O2
-
 ?
spermidine + O2 + H2O
-
 Kocuria rosea 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 no liberation of ammonia ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Kocuria rosea
50
-
 rapid inactivation at Kocuria rosea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 putrescine Kocuria rosea
8.5
-
 spermidine Kocuria rosea

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol of FAD per mol of enzyme Kocuria rosea