BRENDA - Enzyme Database
show all sequences of 1.4.2.1

Glycine-cytochrome c reductase from Nitrobacter agilis

Sanders, H.K.; Becker, G.E.; Nason, A.; J. Biol. Chem. 247, 2015-2025 (1972) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
55% inhibition at 1 mM
Nitrobacter winogradskyi
cytochrome c
above 0.15 mM
Nitrobacter winogradskyi
diphosphate
buffer, 10 mM
Nitrobacter winogradskyi
EDTA
64% inhibition at 7.1 mM
Nitrobacter winogradskyi
hydrazine
-
Nitrobacter winogradskyi
hydroxylamine
-
Nitrobacter winogradskyi
KCl
80% inhibition at 75 mM
Nitrobacter winogradskyi
KCN
47% inhibition at 7.1 mM
Nitrobacter winogradskyi
KNO3
80% inhibition at 75 mM
Nitrobacter winogradskyi
MgCl2
80% inhibition at 40 mM
Nitrobacter winogradskyi
NaCl
80% inhibition at 75 mM
Nitrobacter winogradskyi
phosphate
buffer, 100 mM
Nitrobacter winogradskyi
pyridoxal 5'-phosphate
up to 80% inhibition at 0.2-2 mM due to reaction with glycine
Nitrobacter winogradskyi
pyruvate
70% inhibition at 10 mM and 20% inhibition at 3 mM
Nitrobacter winogradskyi
Quinacrine hydrochloride
0.3-1.0 mM
Nitrobacter winogradskyi
Sodium azide
10% inhibition at 2 mM
Nitrobacter winogradskyi
sodium barbital
buffer, 100 mM, pH 8.0
Nitrobacter winogradskyi
Tris
buffer, 100 mM, pH 8.0
Nitrobacter winogradskyi
Urea
30% inhibition at 50 mM
Nitrobacter winogradskyi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
ferricytochrome c
-
Nitrobacter winogradskyi
0.8
-
glycine
-
Nitrobacter winogradskyi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
gel filtration, sucrose density gradient centrifugation
Nitrobacter winogradskyi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glycine + ferricytochrome c
Nitrobacter winogradskyi
-
glyoxylate + ferrocytochrome c + NH3
-
Nitrobacter winogradskyi
ir
Organism
Organism
UniProt
Commentary
Textmining
Nitrobacter winogradskyi
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Nitrobacter winogradskyi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.1
-
-
Nitrobacter winogradskyi
Storage Stability
Storage Stability
Organism
4°C, 4-10 weeks, 30-50% loss of activity
Nitrobacter winogradskyi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glycine + ferricytochrome c
-
391704
Nitrobacter winogradskyi
glyoxylate + ferrocytochrome c + NH3
-
391704
Nitrobacter winogradskyi
ir
glycine + ferricytochrome c
highly specific
391704
Nitrobacter winogradskyi
glyoxylate + ferrocytochrome c + NH3
-
391704
Nitrobacter winogradskyi
ir
glycine + H2O + ferricytochrome c
-
391704
Nitrobacter winogradskyi
glyoxylate + NH3 + ferrocytochrome c + H+
-
-
-
r
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
4-10 weeks, 30-50% loss of activity
Nitrobacter winogradskyi
70
-
5 min: 75% loss of activity, 2 min: 50% loss of activity
Nitrobacter winogradskyi
80
-
2 min: 100% loss of activity
Nitrobacter winogradskyi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
6.9
in potassium phosphate or diphosphate buffer
Nitrobacter winogradskyi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
55% inhibition at 1 mM
Nitrobacter winogradskyi
cytochrome c
above 0.15 mM
Nitrobacter winogradskyi
diphosphate
buffer, 10 mM
Nitrobacter winogradskyi
EDTA
64% inhibition at 7.1 mM
Nitrobacter winogradskyi
hydrazine
-
Nitrobacter winogradskyi
hydroxylamine
-
Nitrobacter winogradskyi
KCl
80% inhibition at 75 mM
Nitrobacter winogradskyi
KCN
47% inhibition at 7.1 mM
Nitrobacter winogradskyi
KNO3
80% inhibition at 75 mM
Nitrobacter winogradskyi
MgCl2
80% inhibition at 40 mM
Nitrobacter winogradskyi
NaCl
80% inhibition at 75 mM
Nitrobacter winogradskyi
phosphate
buffer, 100 mM
Nitrobacter winogradskyi
pyridoxal 5'-phosphate
up to 80% inhibition at 0.2-2 mM due to reaction with glycine
Nitrobacter winogradskyi
pyruvate
70% inhibition at 10 mM and 20% inhibition at 3 mM
Nitrobacter winogradskyi
Quinacrine hydrochloride
0.3-1.0 mM
Nitrobacter winogradskyi
Sodium azide
10% inhibition at 2 mM
Nitrobacter winogradskyi
sodium barbital
buffer, 100 mM, pH 8.0
Nitrobacter winogradskyi
Tris
buffer, 100 mM, pH 8.0
Nitrobacter winogradskyi
Urea
30% inhibition at 50 mM
Nitrobacter winogradskyi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
ferricytochrome c
-
Nitrobacter winogradskyi
0.8
-
glycine
-
Nitrobacter winogradskyi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
gel filtration, sucrose density gradient centrifugation
Nitrobacter winogradskyi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glycine + ferricytochrome c
Nitrobacter winogradskyi
-
glyoxylate + ferrocytochrome c + NH3
-
Nitrobacter winogradskyi
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nitrobacter winogradskyi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.1
-
-
Nitrobacter winogradskyi
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 4-10 weeks, 30-50% loss of activity
Nitrobacter winogradskyi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glycine + ferricytochrome c
-
391704
Nitrobacter winogradskyi
glyoxylate + ferrocytochrome c + NH3
-
391704
Nitrobacter winogradskyi
ir
glycine + ferricytochrome c
highly specific
391704
Nitrobacter winogradskyi
glyoxylate + ferrocytochrome c + NH3
-
391704
Nitrobacter winogradskyi
ir
glycine + H2O + ferricytochrome c
-
391704
Nitrobacter winogradskyi
glyoxylate + NH3 + ferrocytochrome c + H+
-
-
-
r
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
4-10 weeks, 30-50% loss of activity
Nitrobacter winogradskyi
70
-
5 min: 75% loss of activity, 2 min: 50% loss of activity
Nitrobacter winogradskyi
80
-
2 min: 100% loss of activity
Nitrobacter winogradskyi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
6.9
in potassium phosphate or diphosphate buffer
Nitrobacter winogradskyi
Other publictions for EC 1.4.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
391704
Sanders
Glycine-cytochrome c reductase ...
Nitrobacter winogradskyi
J. Biol. Chem.
247
2015-2025
1972
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19
2
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1
1
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3
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1
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1
1
3
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3
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1
-
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19
-
2
-
-
1
1
-
-
-
1
-
-
1
1
3
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3
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1
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