BRENDA - Enzyme Database
show all sequences of 1.4.1.B5

Glutamate synthase, but not GABA shunt enzymes, contributes to nitrogen metabolism of the sheep abomasal nematode parasites Haemonchus contortus and Teladorsagia circumcincta

Umair, S.; Bland, R.J.; Simpson, H.V.; Exp. Parasitol. 127, 9-13 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NH4Cl
activates
Teladorsagia circumcincta
Inhibitors
Inhibitors
Commentary
Organism
Structure
azaserine
strong inhibition
Haemonchus contortus
azaserine
strong inhibition
Teladorsagia circumcincta
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
2-oxoglutarate
pH 7.5, 30°C, L3 stage enzyme
Teladorsagia circumcincta
0.6
-
L-glutamine
pH 7.5, 30°C, L3 stage enzyme
Teladorsagia circumcincta
0.8
-
2-oxoglutarate
pH 7.5, 30°C, adult worm enzyme
Teladorsagia circumcincta
0.9
-
2-oxoglutarate
pH 7.5, 30°C
Haemonchus contortus
1
-
L-glutamine
pH 7.5, 30°C
Haemonchus contortus
1.5
-
L-glutamine
pH 7.5, 30°C, adult worm enzyme
Teladorsagia circumcincta
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NH4Cl
activates
Haemonchus contortus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 L-glutamate + NAD+
Haemonchus contortus
-
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
r
2 L-glutamate + NAD+
Teladorsagia circumcincta
-
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
r
2 L-glutamate + NADP+
Haemonchus contortus
-
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
r
2 L-glutamate + NADP+
Teladorsagia circumcincta
-
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Haemonchus contortus
-
-
-
Teladorsagia circumcincta
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
larva
L3 stage
Teladorsagia circumcincta
-
additional information
no activity in L3 stage
Haemonchus contortus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.024
0.041
pH 7.5, 30°C, L3 stage
Teladorsagia circumcincta
0.033
0.059
pH 7.5, 30°C, adult worm
Haemonchus contortus
0.051
0.095
pH 7.5, 30°C, adult worm
Teladorsagia circumcincta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2 L-glutamate + NAD+
-
724925
Haemonchus contortus
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
-
r
2 L-glutamate + NAD+
-
724925
Teladorsagia circumcincta
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
-
r
2 L-glutamate + NADP+
-
724925
Haemonchus contortus
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
-
r
2 L-glutamate + NADP+
-
724925
Teladorsagia circumcincta
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
GOGAT
-
Haemonchus contortus
GOGAT
-
Teladorsagia circumcincta
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Haemonchus contortus
30
-
assay at
Teladorsagia circumcincta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Haemonchus contortus
7.5
-
-
Teladorsagia circumcincta
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Haemonchus contortus
NAD+
-
Teladorsagia circumcincta
NADH
-
Haemonchus contortus
NADH
-
Teladorsagia circumcincta
NADP+
-
Haemonchus contortus
NADP+
-
Teladorsagia circumcincta
NADPH
-
Haemonchus contortus
NADPH
-
Teladorsagia circumcincta
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NH4Cl
activates
Teladorsagia circumcincta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Haemonchus contortus
NAD+
-
Teladorsagia circumcincta
NADH
-
Haemonchus contortus
NADH
-
Teladorsagia circumcincta
NADP+
-
Haemonchus contortus
NADP+
-
Teladorsagia circumcincta
NADPH
-
Haemonchus contortus
NADPH
-
Teladorsagia circumcincta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
azaserine
strong inhibition
Haemonchus contortus
azaserine
strong inhibition
Teladorsagia circumcincta
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
2-oxoglutarate
pH 7.5, 30°C, L3 stage enzyme
Teladorsagia circumcincta
0.6
-
L-glutamine
pH 7.5, 30°C, L3 stage enzyme
Teladorsagia circumcincta
0.8
-
2-oxoglutarate
pH 7.5, 30°C, adult worm enzyme
Teladorsagia circumcincta
0.9
-
2-oxoglutarate
pH 7.5, 30°C
Haemonchus contortus
1
-
L-glutamine
pH 7.5, 30°C
Haemonchus contortus
1.5
-
L-glutamine
pH 7.5, 30°C, adult worm enzyme
Teladorsagia circumcincta
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NH4Cl
activates
Haemonchus contortus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 L-glutamate + NAD+
Haemonchus contortus
-
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
r
2 L-glutamate + NAD+
Teladorsagia circumcincta
-
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
r
2 L-glutamate + NADP+
Haemonchus contortus
-
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
r
2 L-glutamate + NADP+
Teladorsagia circumcincta
-
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
larva
L3 stage
Teladorsagia circumcincta
-
additional information
no activity in L3 stage
Haemonchus contortus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.024
0.041
pH 7.5, 30°C, L3 stage
Teladorsagia circumcincta
0.033
0.059
pH 7.5, 30°C, adult worm
Haemonchus contortus
0.051
0.095
pH 7.5, 30°C, adult worm
Teladorsagia circumcincta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2 L-glutamate + NAD+
-
724925
Haemonchus contortus
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
-
r
2 L-glutamate + NAD+
-
724925
Teladorsagia circumcincta
L-glutamine + 2-oxoglutarate + NADH + H+
-
-
-
r
2 L-glutamate + NADP+
-
724925
Haemonchus contortus
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
-
r
2 L-glutamate + NADP+
-
724925
Teladorsagia circumcincta
L-glutamine + 2-oxoglutarate + NADPH + H+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Haemonchus contortus
30
-
assay at
Teladorsagia circumcincta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Haemonchus contortus
7.5
-
-
Teladorsagia circumcincta
Other publictions for EC 1.4.1.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724925
Umair
Glutamate synthase, but not GA ...
Haemonchus contortus, Teladorsagia circumcincta
Exp. Parasitol.
127
9-13
2011
1
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-
-
-
-
2
6
-
1
-
4
-
2
-
-
-
-
-
2
3
-
4
-
2
2
-
-
-
2
-
-
8
-
-
-
1
-
-
8
-
-
-
-
2
-
6
-
1
-
4
-
-
-
-
-
2
3
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-