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show all sequences of 1.4.1.B4

Alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota

Hanfrey, C.C.; Pearson, B.M.; Hazeldine, S.; Lee, J.; Gaskin, D.J.; Woster, P.M.; Phillips, M.A.; Michael, A.J.; J. Biol. Chem. 286, 43301-43312 (2011)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
deletion of the gene encoding carboxynorspermidine dehydrogenase. The CASDH gene deletion strain grows normally
Campylobacter jejuni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
Campylobacter jejuni
-
carboxynorspermidine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni
A8FJX8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
-
719993
Campylobacter jejuni
carboxynorspermidine + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Campylobacter jejuni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Campylobacter jejuni
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
deletion of the gene encoding carboxynorspermidine dehydrogenase. The CASDH gene deletion strain grows normally
Campylobacter jejuni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
Campylobacter jejuni
-
carboxynorspermidine + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
-
719993
Campylobacter jejuni
carboxynorspermidine + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the enzyme is found in diverse bacterial phyla, overview. orthologues encoding carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase are found clustered with an array of diverse putrescine biosynthetic genes in different bacterial genomes, consistent with a role in spermidine, rather than sym-norspermidine biosynthesis
Campylobacter jejuni
malfunction
the CASDH gene deletion strain grows normally
Campylobacter jejuni
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is found in diverse bacterial phyla, overview. orthologues encoding carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase are found clustered with an array of diverse putrescine biosynthetic genes in different bacterial genomes, consistent with a role in spermidine, rather than sym-norspermidine biosynthesis
Campylobacter jejuni
malfunction
the CASDH gene deletion strain grows normally
Campylobacter jejuni
Other publictions for EC 1.4.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719993
Hanfrey
Alternative spermidine biosynt ...
Campylobacter jejuni
J. Biol. Chem.
286
43301-43312
2011
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1
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2
2
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712623
Nakao
Purification and some properti ...
Vibrio alginolyticus
J. Gen. Microbiol.
137
1737-1742
1991
2
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1
8
4
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2
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1
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8
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2
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