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Literature summary for 1.4.1.9 extracted from

  • Ouyang, S.; Li, X.; Sun, X.; Ouyang, J.; Yong, Q.
    A thermostable leucine dehydrogenase from Bacillus coagulans NL01 Expression, purification and characterization (2020), Process Biochem., 90, 89-96 .
No PubMed abstract available
Search for more literature for 1.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.37
-
 2-oxopentanoate pH 8, 50°C Weizmannia coagulans

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans
-
-
-
Weizmannia coagulans NL01
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1338
-
 pH 8, 50°C Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxopentanoate + NH3 + NADH + H+
-
 Weizmannia coagulans L-2-aminopentanoate + H2O + NAD+
-
 ?
2-oxopentanoate + NH3 + NADH + H+
-
 Weizmannia coagulans NL01 L-2-aminopentanoate + H2O + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, calculated from sequence Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 72 h, almost 50% residual activity Weizmannia coagulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.125
-
 2-oxopentanoate pH 8, 50°C Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Weizmannia coagulans

pI Value

Organism Comment pI Value Maximum pI Value
Weizmannia coagulans calculated from sequence
-
 5.8