Literature summary for 1.4.1.9 extracted from

Jiang, W.; Sun, D.; Lu, J.; Wang, Y.; Wang, S.; Zhang, Y.; Fang, B.
A cold-adapted leucine dehydrogenase from marine bacterium Alcanivorax dieselolei Characterization and l-tert-leucine production (2016), Eng. Life Sci., 16, 283-289 .

No PubMed abstract available

Search for more literature for 1.4.1.9

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	NADH	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei
100	-	3,3-dimethyl-2-oxobutanoate	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei

Organism

Organism	UniProt	Comment	Textmining
Alcanivorax dieselolei	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.88	-	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,3-dimethyl-2-oxobutanoate + NH3 + NADH	-	Alcanivorax dieselolei	L-tert-Leu + H2O + NAD+	-	?

Subunits

Subunits	Comment	Organism
?	x * 36700, calculated	Alcanivorax dieselolei

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Alcanivorax dieselolei

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
-	37	more than 90% of maximum activity	Alcanivorax dieselolei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.1	-	NADH	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei
10.4	-	3,3-dimethyl-2-oxobutanoate	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Alcanivorax dieselolei

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	8	-	Alcanivorax dieselolei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.1	-	3,3-dimethyl-2-oxobutanoate	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei
11	-	NADH	pH 7.0, temperature not specified in the publication	Alcanivorax dieselolei

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Release 2023.1 (January 2023)