Literature summary for 1.4.1.9 extracted from

Zhu, L.; Wu, Z.; Jin, J.M.; Tang, S.Y.
Directed evolution of leucine dehydrogenase for improved efficiency of L-tert-leucine synthesis (2016), Appl. Microbiol. Biotechnol., 100, 5805-5813 .

Search for more literature for 1.4.1.9

Application

Application	Comment	Organism
synthesis	high-throughput screening method for L-tert-leucine synthesis and directed evolution strategy to engineer LeuDH for improved efficiency of L-tert-leucine synthesis	Lysinibacillus sphaericus

Protein Variants

Protein Variants	Comment	Organism
A43V/D124E	mutant shows improved efficiency of L-tert-leucine synthesis, 5fold increase in catalyic efficiency compared to wild-type	Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	NADH	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus
0.2	-	NADH	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus
2.6	-	3,3-dimethyl-2-oxobutanoate	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus
12	-	3,3-dimethyl-2-oxobutanoate	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	Q76GS2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,3-dimethyl-2-oxobutanoate + NH3 + NADH	-	Lysinibacillus sphaericus	L-tert-Leu + H2O + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
LeuDH	-	Lysinibacillus sphaericus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
31	-	3,3-dimethyl-2-oxobutanoate	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus
34	-	3,3-dimethyl-2-oxobutanoate	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus
96	-	NADH	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus
110	-	NADH	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.6	-	3,3-dimethyl-2-oxobutanoate	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus
13	-	3,3-dimethyl-2-oxobutanoate	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus
480	-	NADH	wild-type, pH 10.0, 30°C	Lysinibacillus sphaericus
2800	-	NADH	mutant A43V/D124E , pH 10.0, 30°C	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)