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Literature summary for 1.4.1.9 extracted from

  • Katoh, R.; Nagata, S.; Misono, H.
    Cloning and sequencing of the leucine dehydrogenase gene from Bacillus sphaericus IFO 3525 and importance of the C-terminal region for the enzyme activity (2003), J. Mol. Catal. B, 23, 239-247.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lysinibacillus sphaericus

Protein Variants

Protein Variants Comment Organism
LeuDEL4 4 C-terminal amino acids deleted, mutant enzyme is a dimer Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
NAD+ native enzyme Lysinibacillus sphaericus
1.25
-
NAD+ mutant enzyme LeuDEL4 Lysinibacillus sphaericus
3.3
-
L-Leu native enzyme Lysinibacillus sphaericus
3.8
-
L-Val native enzyme Lysinibacillus sphaericus
11
-
L-Leu mutant enzyme LeuDEL4 Lysinibacillus sphaericus
25
-
L-Val mutant enzyme LeuDEL4 Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
8 * 43000, recombinant native enzyme, SDS-PAGE Lysinibacillus sphaericus
82000
-
mutant enzyme LeuDEL4, gel filtration Lysinibacillus sphaericus
340000
-
recombinant native enzyme, gel filtration Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
IFO 3525
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
71.7
-
-
Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Val + H2O + NAD+
-
Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

Subunits

Subunits Comment Organism
dimer mutant enzyme LeuDEL4 Lysinibacillus sphaericus
octamer 8 * 43000, recombinant native enzyme, SDS-PAGE Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to, mutant enzyme LeuDEL4 Lysinibacillus sphaericus
55
-
stable up to, native enzyme Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
mutant enzyme LeuDEL4 Lysinibacillus sphaericus
11
-
native enzyme Lysinibacillus sphaericus

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
mutant enzyme LeuDEL4 is stable Lysinibacillus sphaericus
9.5 10.5 native enzyme is stable Lysinibacillus sphaericus