Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lysinibacillus sphaericus |
Protein Variants | Comment | Organism |
---|---|---|
LeuDEL4 | 4 C-terminal amino acids deleted, mutant enzyme is a dimer | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
NAD+ | native enzyme | Lysinibacillus sphaericus | |
1.25 | - |
NAD+ | mutant enzyme LeuDEL4 | Lysinibacillus sphaericus | |
3.3 | - |
L-Leu | native enzyme | Lysinibacillus sphaericus | |
3.8 | - |
L-Val | native enzyme | Lysinibacillus sphaericus | |
11 | - |
L-Leu | mutant enzyme LeuDEL4 | Lysinibacillus sphaericus | |
25 | - |
L-Val | mutant enzyme LeuDEL4 | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
8 * 43000, recombinant native enzyme, SDS-PAGE | Lysinibacillus sphaericus |
82000 | - |
mutant enzyme LeuDEL4, gel filtration | Lysinibacillus sphaericus |
340000 | - |
recombinant native enzyme, gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
IFO 3525 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.7 | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Val + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | mutant enzyme LeuDEL4 | Lysinibacillus sphaericus |
octamer | 8 * 43000, recombinant native enzyme, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to, mutant enzyme LeuDEL4 | Lysinibacillus sphaericus |
55 | - |
stable up to, native enzyme | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
mutant enzyme LeuDEL4 | Lysinibacillus sphaericus |
11 | - |
native enzyme | Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
mutant enzyme LeuDEL4 is stable | Lysinibacillus sphaericus |
9.5 | 10.5 | native enzyme is stable | Lysinibacillus sphaericus |