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Literature summary for 1.4.1.9 extracted from

  • Nagata, S.; Misono, H.; Nagasaki, S.; Esaki, N.; Tanaka, H.; Soda, K.
    Gene cloning, purification, and characterization of the highly thermostable leucine dehydrogenase of bacillus sp. (1990), J. Ferment. Bioeng., 69, 199-203.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
NAD+
-
Bacillus sp. (in: Bacteria)
2.8
-
L-Leu
-
Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
6 * 47000, SDS-PAGE Bacillus sp. (in: Bacteria)
280000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
DSM 730
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ile + H2O + NAD+
-
Bacillus sp. (in: Bacteria) 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus sp. (in: Bacteria) 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Val + H2O + NAD+
-
Bacillus sp. (in: Bacteria) 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 47000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 5.4-10.3, 10 min, stable Bacillus sp. (in: Bacteria)
80
-
10 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
reductive amination of 4-methyl-2-oxopentanoate Bacillus sp. (in: Bacteria)
11.3
-
oxidative deamination of L-Leu Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.4 10.3 55°C, 10 min, stable Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Bacillus sp. (in: Bacteria)
additional information no activity with NADP+ Bacillus sp. (in: Bacteria)
NAD+
-
Bacillus sp. (in: Bacteria)
NADH
-
Bacillus sp. (in: Bacteria)