Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
2.8 | - |
L-Leu | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
6 * 47000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
280000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
DSM 730 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ile + H2O + NAD+ | - |
Bacillus sp. (in: Bacteria) | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus sp. (in: Bacteria) | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Val + H2O + NAD+ | - |
Bacillus sp. (in: Bacteria) | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 47000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 5.4-10.3, 10 min, stable | Bacillus sp. (in: Bacteria) |
80 | - |
10 min, stable | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
reductive amination of 4-methyl-2-oxopentanoate | Bacillus sp. (in: Bacteria) |
11.3 | - |
oxidative deamination of L-Leu | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 10.3 | 55°C, 10 min, stable | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Bacillus sp. (in: Bacteria) | |
additional information | no activity with NADP+ | Bacillus sp. (in: Bacteria) | |
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
NADH | - |
Bacillus sp. (in: Bacteria) |