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Literature summary for 1.4.1.9 extracted from

  • Livesey, G.; Lund, P.
    Isolation and characterization of leucine dehydrogenase from Bacillus subtilis (1988), Methods Enzymol., 166, 282-288.
    View publication on PubMed
Search for more literature for 1.4.1.9

Inhibitors

Inhibitors Comment Organism Structure
L-Leu competitive inhibition of the reductive amination of 4-methylthio-2-oxobutanoate Bacillus subtilis
Mn2+ inhibits oxidative deamination Bacillus subtilis
NH4+ inhibits oxidative deamination Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 4-methyl-2-oxopentanoate
-
 Bacillus subtilis
0.9
-
 2-oxo-3-methylpentanoate
-
 Bacillus subtilis
1.33
-
 L-Leu
-
 Bacillus subtilis
1.5
-
 NAD+
-
 Bacillus subtilis
2.4
-
 L-Ile
-
 Bacillus subtilis
3.3
-
 3-methyl-2-oxobutanoate
-
 Bacillus subtilis
3.4
-
 L-Val
-
 Bacillus subtilis
6.7
-
 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis
25
-
 L-Met
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.23
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
-
 Bacillus subtilis L-methionine + H2O + NAD+
-
 r
L-2-aminobutanoate + H2O + NAD+
-
 Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
 ?
L-Ile + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Met + H2O + NAD+
-
 Bacillus subtilis 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
 r
L-norleucine + H2O + NAD+
-
 Bacillus subtilis 2-oxohexanoate + NH3 + NADH
-
 ?
L-Val + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 reductive amination of 4-methyl-2-oxobutanoate Bacillus subtilis
11
-
 oxidative deamination of L-Leu Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NADH
-
 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 L-Leu competitive inhibition of the reductive amination of 4-methylthio-2-oxobutanoate Bacillus subtilis