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Literature summary for 1.4.1.9 extracted from

  • Zink, M.W.; Sanwal, B.D.
    The distribution and substrate specificity of L-Leucine dehydrogenase (1962), Arch. Biochem. Biophys., 99, 72-77.
No PubMed abstract available
Search for more literature for 1.4.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 NADH
-
 Bacillus subtilis
0.12
-
 NADH
-
 Bacillus cereus
0.16
-
 NAD+
-
 Bacillus subtilis
0.16
-
 NAD+
-
 Bacillus cereus
2.2
-
 3-methyl-2-oxobutanoate
-
 Bacillus subtilis
2.2
-
 3-methyl-2-oxobutanoate
-
 Bacillus cereus
3.3
-
 3-methyl-2-oxobutanoate
-
 Bacillus subtilis
5.2
-
 L-Ile
-
 Bacillus subtilis
5.2
-
 L-Ile
-
 Bacillus cereus
6.2
-
 L-Leu
-
 Bacillus subtilis
6.2
-
 L-Leu
-
 Bacillus cereus
13
-
 NH4+
-
 Bacillus subtilis
13
-
 NH4+
-
 Bacillus cereus
20
-
 L-Val
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus mycoides
-
-
-
Bacillus niger
-
-
-
Bacillus pumilus
-
-
-
Bacillus subtilis
-
-
-
Lysinibacillus sphaericus
-
-
-
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
spore
-
 Bacillus subtilis
-
spore
-
 Bacillus cereus
-
vegetative
-
 Bacillus subtilis
-
vegetative
-
 Bacillus cereus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+
-
 Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-2-aminobutanoate + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-2-aminobutanoate + H2O + NAD+
-
 Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-Ile + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Ile + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Ile + H2O + NAD+
-
 Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Priestia megaterium 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus pumilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus mycoides 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-Leu + H2O + NAD+
-
 Bacillus niger 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
L-norleucine + H2O + NAD+
-
 Bacillus subtilis 2-oxohexanoate + NH3 + NADH
-
 ?
L-norleucine + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
 ?
L-norleucine + H2O + NAD+
-
 Bacillus cereus 2-oxohexanoate + NH3 + NADH
-
 ?
L-Val + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r
L-Val + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r
L-Val + H2O + NAD+
-
 Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NAD+
-
 Priestia megaterium
NAD+
-
 Lysinibacillus sphaericus
NAD+
-
 Bacillus cereus
NAD+
-
 Bacillus pumilus
NAD+
-
 Bacillus mycoides
NAD+
-
 Bacillus niger
NADH
-
 Bacillus subtilis
NADH
-
 Priestia megaterium
NADH
-
 Lysinibacillus sphaericus
NADH
-
 Bacillus cereus
NADH
-
 Bacillus pumilus
NADH
-
 Bacillus mycoides
NADH
-
 Bacillus niger