L-Amino acid dehydrogenases in Bacillus subtilis spores

Nitta, Y.; Yasuda, Y.; Tochikubo, K.; Hachisuka, Y.; J. Bacteriol. 117, 588-592 (1974)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Zn2+

-

Bacillus subtilis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.16

-

NAD+

(+ L-isoleucine)

Bacillus subtilis

2.2

-

L-isoleucine

-

Bacillus subtilis

25

-

L-valine

-

Bacillus subtilis

33

-

NAD+

(+ L-valine)

Bacillus subtilis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

L-Amino acid + H2O + NAD+

Bacillus subtilis

-

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

L-Amino acid + H2O + NAD+

aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine

12940

Bacillus subtilis

2-Oxo acid + NH3 + NADH

-

-

-

?

L-Amino acid + H2O + NAD+

-

12940

Bacillus subtilis

?

-

-

-

?

L-isoleucine + H2O + NAD+

-

12940

Bacillus subtilis

3-methyl-2-oxopentanoate + NH3 + NADH + H+

-

-

-

r

L-valine + H2O + NAD+

-

12940

Bacillus subtilis

3-methyl-2-oxobutanoate + NH3 + NADH+ H+

-

-

-

r

Cofactor

Commentary

Organism

Structure

NAD+

cannot be replaced by NADP+

Bacillus subtilis

Cofactor

Commentary

Organism

Structure

NAD+

cannot be replaced by NADP+

Bacillus subtilis

Inhibitors

Commentary

Organism

Structure

Zn2+

-

Bacillus subtilis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.16

-

NAD+

(+ L-isoleucine)

Bacillus subtilis

2.2

-

L-isoleucine

-

Bacillus subtilis

25

-

L-valine

-

Bacillus subtilis

33

-

NAD+

(+ L-valine)

Bacillus subtilis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

L-Amino acid + H2O + NAD+

Bacillus subtilis

-

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

L-Amino acid + H2O + NAD+

aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine

12940

Bacillus subtilis

2-Oxo acid + NH3 + NADH

-

-

-

?

L-Amino acid + H2O + NAD+

-

12940

Bacillus subtilis

?

-

-

-

?

L-isoleucine + H2O + NAD+

-

12940

Bacillus subtilis

3-methyl-2-oxopentanoate + NH3 + NADH + H+

-

-

-

r

L-valine + H2O + NAD+

-

12940

Bacillus subtilis

3-methyl-2-oxobutanoate + NH3 + NADH+ H+

-

-

-

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

704871

Schriek

Detection of an L-amino acid d ...

Synechocystis sp.

J. Exp. Bot.

60

1035-1046

2009

-

-

-

-

-

-

13

1

1

1

1

-

-

7

-

-

-

-

-

-

-

-

19

-

2

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

13

-

1

1

1

1

-

-

-

-

-

-

-

-

-

19

-

-

-

-

-

1

-

-

-

1

-

-

1

-

-

687209

Mohammadi

Preliminary report of NAD+-dep ...

Citrobacter sp., Proteus sp., Pseudomonas sp.

Iran. Biomed. J.

11

131-135

2007

-

-

-

-

-

-

-

4

-

-

-

-

-

8

-

-

-

-

-

-

5

-

14

-

3

-

-

-

4

-

-

-

3

-

-

-

-

-

-

3

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

5

-

14

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

12940

Nitta

L-Amino acid dehydrogenases in ...

Bacillus subtilis

J. Bacteriol.

117

588-592

1974

-

-

-

-

-

-

1

4

-

-

1

1

-

1

-

-

1

-

-

1

-

-

4

-

-

1

-

1

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

4

-

-

1

1

-

-

-

1

-

1

-

-

4

-

1

-

1

-

1

-

-

-

-

-

-

-

-

-