BRENDA - Enzyme Database
show all sequences of 1.4.1.5

L-Amino acid dehydrogenases in Bacillus subtilis spores

Nitta, Y.; Yasuda, Y.; Tochikubo, K.; Hachisuka, Y.; J. Bacteriol. 117, 588-592 (1974)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Zn2+
-
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
(+ L-isoleucine)
Bacillus subtilis
2.2
-
L-isoleucine
-
Bacillus subtilis
25
-
L-valine
-
Bacillus subtilis
33
-
NAD+
(+ L-valine)
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
290000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-Amino acid + H2O + NAD+
Bacillus subtilis
-
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Bacillus subtilis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spore
resting
Bacillus subtilis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-Amino acid + H2O + NAD+
aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine
12940
Bacillus subtilis
2-Oxo acid + NH3 + NADH
-
-
-
?
L-Amino acid + H2O + NAD+
-
12940
Bacillus subtilis
?
-
-
-
?
L-isoleucine + H2O + NAD+
-
12940
Bacillus subtilis
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
L-valine + H2O + NAD+
-
12940
Bacillus subtilis
3-methyl-2-oxobutanoate + NH3 + NADH+ H+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
10 min, 25-35% loss of activity
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.7
-
L-valine, L-isoleucine
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Zn2+
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
(+ L-isoleucine)
Bacillus subtilis
2.2
-
L-isoleucine
-
Bacillus subtilis
25
-
L-valine
-
Bacillus subtilis
33
-
NAD+
(+ L-valine)
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
290000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-Amino acid + H2O + NAD+
Bacillus subtilis
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bacillus subtilis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spore
resting
Bacillus subtilis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-Amino acid + H2O + NAD+
aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine
12940
Bacillus subtilis
2-Oxo acid + NH3 + NADH
-
-
-
?
L-Amino acid + H2O + NAD+
-
12940
Bacillus subtilis
?
-
-
-
?
L-isoleucine + H2O + NAD+
-
12940
Bacillus subtilis
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
L-valine + H2O + NAD+
-
12940
Bacillus subtilis
3-methyl-2-oxobutanoate + NH3 + NADH+ H+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
20
-
10 min, 25-35% loss of activity
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.7
-
L-valine, L-isoleucine
Bacillus subtilis
Other publictions for EC 1.4.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704871
Schriek
Detection of an L-amino acid d ...
Synechocystis sp.
J. Exp. Bot.
60
1035-1046
2009
-
-
-
-
-
-
13
1
1
1
1
-
-
7
-
-
-
-
-
-
-
-
19
-
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
13
-
1
1
1
1
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
687209
Mohammadi
Preliminary report of NAD+-dep ...
Citrobacter sp., Proteus sp., Pseudomonas sp.
Iran. Biomed. J.
11
131-135
2007
-
-
-
-
-
-
-
4
-
-
-
-
-
8
-
-
-
-
-
-
5
-
14
-
3
-
-
-
4
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
5
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
12940
Nitta
L-Amino acid dehydrogenases in ...
Bacillus subtilis
J. Bacteriol.
117
588-592
1974
-
-
-
-
-
-
1
4
-
-
1
1
-
1
-
-
1
-
-
1
-
-
4
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
1
1
-
-
-
1
-
1
-
-
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-