Application | Comment | Organism |
---|---|---|
agriculture | enzyme TrGDH is a promising candidate gene for maintaining or improving yields in crop plants via genetic engineering | Trichurus sp. |
biotechnology | enzyme TrGDH is a promising candidate gene for maintaining or improving yields in crop plants via genetic engineering | Trichurus sp. |
Cloned (Comment) | Organism |
---|---|
TrGDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant FLAG-tagged enzyme expression in Oryza sativa cv. Kitaake under control of the 35S CaMV 35S promoter, semiquantitative RT-PCR and quantitative real-time PCR enzyme expression analysis | Trichurus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of an NADP(H)-dependent glutamate dehydrogenase gene, TrGDH, from Trichurus sp. improves nitrogen assimilation, growth status, and grain weight per plant in rice, phenotype, overview. Compared with the rice GDH (OsGDH4), TrGDH exhibits higher affinity for NH4+ | Trichurus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk kinetics | Trichurus sp. | |
1.48 | - |
NH3 | pH 8.0, 25°C, recombinant enzyme | Trichurus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | Trichurus sp. | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichurus sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Trichurus sp. | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP(H)-dependent glutamate dehydrogenase | - |
Trichurus sp. |
NADP(H)-GDH | - |
Trichurus sp. |
TrGDH | - |
Trichurus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, reductive amination reaction | Trichurus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at, reductive amination reaction | Trichurus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | beta-NADP+ | Trichurus sp. | |
NADPH | beta-NADPH | Trichurus sp. |