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Literature summary for 1.4.1.4 extracted from

  • Wakamatsu, T.; Higashi, C.; Ohmori, T.; Doi, K.; Ohshima, T.
    Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis (2013), Extremophiles, 17, 379-389 .
    View publication on PubMed
Search for more literature for 1.4.1.4

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, 76% residual activity Pyrobaculum calidifontis
isocitrate 10 M, 64% of initial activity Pyrobaculum calidifontis
KCl 100 mM, 45% residual activity Pyrobaculum calidifontis
NaCl 100 mM, 59% residual activity Pyrobaculum calidifontis
oxaloacetate 10 mM, 63% residual activity Pyrobaculum calidifontis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis
0.035
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
1.7
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
2.2
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis
3.4
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 gel filtration Pyrobaculum calidifontis

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MWK6
-
-
Pyrobaculum calidifontis JCM 11548 A3MWK6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADPH + H+ 2.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-2-aminobutanoate + H2O + NADP+
-
 r
2-oxobutanoate + NH3 + NADPH + H+ 2.6% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-2-aminobutanoate + H2O + NADP+
-
 r
2-oxoglutarate + NH3 + NADPH + H+
-
 Pyrobaculum calidifontis L-glutamate + H2O + NADP+
-
 r
2-oxoglutarate + NH3 + NADPH + H+
-
 Pyrobaculum calidifontis JCM 11548 L-glutamate + H2O + NADP+
-
 r
2-oxovalerate + NH3 + NADPH + H+ 4.2% of the activity with 2-oxoglutarate Pyrobaculum calidifontis L-valine + H2O + NADP+
-
 r
2-oxovalerate + NH3 + NADPH + H+ 4.2% of the activity with 2-oxoglutarate Pyrobaculum calidifontis JCM 11548 L-valine + H2O + NADP+
-
 r
L-glutamate + H2O + NADP+
-
 Pyrobaculum calidifontis 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-glutamate + H2O + NADP+
-
 Pyrobaculum calidifontis JCM 11548 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-norvaline + H2O + NADP+ 3% of the activity with L-glutamate Pyrobaculum calidifontis 2-oxopentanoate + NH3 + NADPH + H+
-
 r
L-norvaline + H2O + NADP+ 3% of the activity with L-glutamate Pyrobaculum calidifontis JCM 11548 2-oxopentanoate + NH3 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 47700, calculated Pyrobaculum calidifontis

Synonyms

Synonyms Comment Organism
Pcal_1606
-
 Pyrobaculum calidifontis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.9
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
13
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis
92
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis
95
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
100
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis

Cofactor

Cofactor Comment Organism Structure
NAD+ about 17% of the activity with NADP+ Pyrobaculum calidifontis
NADH about 7% of the activity with NADPH Pyrobaculum calidifontis
NADP+
-
 Pyrobaculum calidifontis
NADPH
-
 Pyrobaculum calidifontis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8
-
 L-glutamate pH 9.5, 50°C Pyrobaculum calidifontis
45
-
 NH3 pH 9.0, 50°C Pyrobaculum calidifontis
56
-
 2-oxoglutarate pH 9.0, 50°C Pyrobaculum calidifontis
280
-
 NADP+ pH 9.5, 50°C Pyrobaculum calidifontis
5400
-
 NADPH pH 9.0, 50°C Pyrobaculum calidifontis