Cloned (Comment) | Organism |
---|---|
gene msmeg_5442, semi-quantitative real-time PCR expression analysis | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | Mycolicibacterium smegmatis | reaction cycle, specificities of forward and reverse reactions, overview | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R3E3 | gene msmeg_5442 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | reaction cycle, specificities of forward and reverse reactions, overview | Mycolicibacterium smegmatis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | reaction cycle, overview | Mycolicibacterium smegmatis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH1 | - |
Mycolicibacterium smegmatis |
NADP+ - dependant-glutamate dehydrogenase | - |
Mycolicibacterium smegmatis |
NADP+-Gdh | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for | Mycolicibacterium smegmatis | |
NADPH | specific for | Mycolicibacterium smegmatis |
Organism | Comment | Expression |
---|---|---|
Mycolicibacterium smegmatis | enzyme expression is upregulated under N2-starvation, overview | up |
General Information | Comment | Organism |
---|---|---|
metabolism | NADP+-GDH is involved in nitrogen assimilation due to a constitutive aminating activity, specific activity of the aminating NADP+-GDH reaction is independent of nitrogen availability, it does not change significantly in response to prolonged exposure to nitrogen limitation, in contrast to the deaminating activity, which is 2fold increased exposed to ammonium starvation conditions. The deaminating reaction changes in response to varying ammonium concentrations and is regulated in response to nitrogen availability. NADP+-GDH is not regulated on the transcriptional level. The enzyme is invovled in the additional nitrogen assimilatory pathway via glutamate dehydrogenase, GDH, regulation of NADP+-GDH specific activity, overview | Mycolicibacterium smegmatis |