Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | 30% stimulation in the presence of 2 mM Mg2+ | Aspergillus terreus | |
GDP | 30% stimulation in the presence of 2 mM Mg2+ | Aspergillus terreus | |
GTP | 30% stimulation in the presence of 2 mM Mg2+ | Aspergillus terreus |
General Stability | Organism |
---|---|
after incubation with chymotrypsin enzyme loses 50% activity within 30 min and a combination of 2-oxoglutarate and NADP+ affords near complete protection against chymotrypsin | Aspergillus niger |
Aspergillus terreus NADP-GDH remains fully active even after 2 h of incubation with chymotrypsin | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-pyridinedicarboxylate | weak inhibitor | Aspergillus terreus | |
2-Methyleneglutarate | weak inhibitor | Aspergillus terreus | |
isophthalate | weak inhibitor | Aspergillus terreus | |
NH4+ | inhibits the oxidative deamination activity as a product, inhibition is non-competitive with respect to L-glutamate | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NADPH | 28°C | Aspergillus niger | |
0.017 | - |
NADP+ | 28°C | Aspergillus niger | |
0.045 | - |
NADP+ | 28°C | Aspergillus terreus | |
0.055 | - |
NADPH | 28°C | Aspergillus terreus | |
0.95 | - |
NH4+ | 28°C | Aspergillus terreus | |
1.05 | - |
NH4+ | 28°C | Aspergillus niger | |
4.78 | - |
2-oxoglutarate | 28°C | Aspergillus niger | |
6 | - |
2-oxoglutarate | 28°C | Aspergillus terreus | |
24.1 | - |
L-glutamate | 28°C | Aspergillus terreus | |
34.6 | - |
L-glutamate | 28°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Aspergillus terreus | |
Zn2+ | forward reaction of GDH from Aspergillus terreus is 80% inhibited by 5 mM ZnCl2 | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Aspergillus terreus |
350000 | - |
FPLC on a Superdex G-75 column | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
GDH from Aspergillus terreus is kinetically distinct from GDH of Aspergilli niger | - |
Aspergillus terreus | - |
GDH from Aspergillus terreus is kinetically distinct from GDH of Aspergilli niger | - |
Purification (Comment) | Organism |
---|---|
purified to homogeneity using novel reactive dye-affinity resins | Aspergillus niger |
purified to homogeneity using novel reactive dye-affinity resins | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger | L-glutamate + NADP+ | - |
? | |
2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus terreus | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Aspergillus niger |
hexamer | 6 x 50000, SDS-PAGE | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
NADP-glutamate dehydrogenase | - |
Aspergillus niger |
NADP-glutamate dehydrogenase | - |
Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Aspergillus niger |
28 | - |
assay at | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Aspergillus terreus |
9.5 | - |
oxidative deamination | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specifically used | Aspergillus niger | |
NADPH | specifically used | Aspergillus terreus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
NH4+ | - |
Aspergillus terreus |