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Literature summary for 1.4.1.4 extracted from
- The crystal structure of Plasmodium falciparum glutamate dehydrogenase, a putative target for novel antimalarial drugs (2005), J. Mol. Biol., 349, 597-607.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | in the parasite protein, several salt-bridges mediate contacts between the subunits, possesses a unique N-terminal extension that does not occur in any other GDH sequence so far studied, design of peptidomimetics capable of disrupting the oligomeric organisation of the parasite enzyme | Plasmodium falciparum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant GDH transformed into competent Escherichia coli PA340 cells, which are glutamate synthase and dehydrogenase-deficient | Plasmodium falciparum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| plate-like crystals of monoclinic space group C2 grown by vapour-diffusion using the sitting-drop method, X-ray crystallography to a resolution of 2.7 A | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion mutant lacking the first 19 amino acid residues, shows unchanged activity and forms hexamers, thus the unique extension does not appear to be essential for catalysis and subunit assembly, and presumably fulfils some other yet unknown function | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NAD(P)+ + H2O | - |
Plasmodium falciparum | 2-oxoglutarate + NAD(P)H + NH3 | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | crystallography | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH | - |
Plasmodium falciparum |
| NADP-dependent glutamate dehydrogenase | - |
Plasmodium falciparum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Plasmodium falciparum | |
| NADPH | - |
Plasmodium falciparum | |
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