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Literature summary for 1.4.1.4 extracted from

  • Lawit, S.J.; Miller, P.W.; Dunn, W.I.; Mirabile, J.S.; Schmidt, R.R.
    Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers (2003), Plant Mol. Biol., 52, 605-616.
    View publication on PubMed
Search for more literature for 1.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA encoding alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus, DELTA40N, expression in Escherichia coli Chlorella sorokiniana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADP+ pH 7.0, 38.5°C, DELTA40N-homohexamer Chlorella sorokiniana
0.027
-
 NADP+ pH 7.0, 38.5°C, alpha-homohexamer Chlorella sorokiniana
0.028
-
 NADP+ pH 7.0, 38.5°C, beta-homohexamer Chlorella sorokiniana
0.125
-
 NADPH pH 7.0, 38.5°C, DELTA40N-homohexamer Chlorella sorokiniana
0.14
-
 NADPH pH 7.0, 38.5°C, alpha-homohexamer Chlorella sorokiniana
0.26
-
 NADPH pH 7.0, 38.5°C, beta-homohexamer Chlorella sorokiniana
3.2
-
 2-oxoglutarate pH 7.0, 38.5°C, DELTA40N-homohexamer Chlorella sorokiniana
5
-
 2-oxoglutarate pH 7.0, 38.5°C, alpha-homohexamer Chlorella sorokiniana
6.6
-
 NH4+ pH 7.0, 38.5°C, alpha-homohexamer Chlorella sorokiniana
6.8
-
 2-oxoglutarate pH 7.0, 38.5°C, beta-homohexamer Chlorella sorokiniana
10.8
-
 NH4+ pH 7.0, 38.5°C, DELTA40N-homohexamer Chlorella sorokiniana
21
-
 L-glutamate pH 7.0, 38.5°C, beta-homohexamer Chlorella sorokiniana
25
-
 L-glutamate pH 7.0, 38.5°C, DELTA40N-homohexamer Chlorella sorokiniana
34
-
 L-glutamate pH 7.0, 38.5°C, alpha-homohexamer Chlorella sorokiniana
80
-
 NH4+ pH 7.0, 38.5°C, beta-homohexamer Chlorella sorokiniana

Organism

Organism UniProt Comment Textmining
Chlorella sorokiniana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus expressed in Escherichia coli Chlorella sorokiniana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
 Chlorella sorokiniana L-glutamate + NADP+
-
 ?
L-glutamate + NADP+ + H2O
-
 Chlorella sorokiniana 2-oxoglutarate + NADPH + NH3
-
 r

Synonyms

Synonyms Comment Organism
NADP-specific glutamate dehydrogenase
-
 Chlorella sorokiniana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 alpha-hexamer and beta-hexamer Chlorella sorokiniana
65
-
 DELTA40N-homohexamer Chlorella sorokiniana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: alpha-homohexamer shows about 65% of maximal activity, beta-homohexamer shows about shows about 70% of maximal activity, DELTA40N-homohexamer shows about 55% of maximal activity. 70°C: alpha-homohexamer shows about 90% of maximal activity, beta-homohexamer shows about 60% of maximal activity, DELTA40N-homohexamer shows about 80% of maximal activity Chlorella sorokiniana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable at Chlorella sorokiniana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Chlorella sorokiniana
NADPH
-
 Chlorella sorokiniana