Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | activates up to 0.1 mM | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | at 1 mM and 10 mM, 30% inhibition and 100% inhibition | Mycolicibacterium smegmatis | |
Hg2+ | at 1 mM, 10% inhibition | Mycolicibacterium smegmatis | |
Zn2+ | over 0.1 mM | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
alpha6, 6 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
245500 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Mycolicibacterium smegmatis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Mycolicibacterium smegmatis CDC46 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis CDC46 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80.2 | - |
reductive amination | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Mycolicibacterium smegmatis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Mycolicibacterium smegmatis CDC46 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reductive amination and oxidative deamination | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination | Mycolicibacterium smegmatis |
8.8 | 9.8 | oxidative deamination | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycolicibacterium smegmatis | |
NADPH | - |
Mycolicibacterium smegmatis |