Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | at 4 mM slight inhibitory | Agaricus bisporus | |
ATP | at 4 mM slight inhibitory | Agaricus bisporus | |
oxaloacetate | at 20 mM slight inhibitory | Agaricus bisporus | |
pyruvate | at 10 mM slight inhibitory | Agaricus bisporus | |
Urea | at 0°C 1 h at 2 M 65% inhibition | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
NADPH | reductive amination at 33°C | Agaricus bisporus | |
0.117 | - |
NADP+ | oxidative deamination at 33°C | Agaricus bisporus | |
2.1 | - |
NH4+ | reductive amination at 33°C | Agaricus bisporus | |
3.2 | - |
2-oxoglutarate | reductive amination at 33°C | Agaricus bisporus | |
27 | - |
L-glutamate | oxidative deamination at 33°C | Agaricus bisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
alpha6, 6 * 48000, SDS-PAGE | Agaricus bisporus |
330000 | - |
gel filtration | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Agaricus bisporus | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.2 | - |
reductive amination at 33°C | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Agaricus bisporus | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Agaricus bisporus | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 48000, SDS-PAGE | Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
reductive amination | Agaricus bisporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
12% activity loss after 1 h | Agaricus bisporus |
37 | - |
72% activity loss after 1 h | Agaricus bisporus |
55 | - |
complete activity loss after 1 h | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
reductive amination | Agaricus bisporus |
9 | - |
oxidative deamination | Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Agaricus bisporus | |
NADPH | - |
Agaricus bisporus |