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Literature summary for 1.4.1.4 extracted from

  • Koike, K.; Hakamada, Y.; Yoshimatsu, T.; Kobayashi, T.; Ito, S.
    NADP-specific glutamate dehydrogenase from alkalophilic Bacillus sp. KSM-635: purification and enzymic properties (1996), Biosci. Biotechnol. Biochem., 60, 1764-1767.
    View publication on PubMed
Search for more literature for 1.4.1.4

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate at 1 mM, 86% inhibition Bacillus sp. (in: Bacteria)
Ag2+ at 1 mM, 60% inhibition Bacillus sp. (in: Bacteria)
Hg2+ at 1 mM, 70% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 NADP+ oxidative deamination at 30°C Bacillus sp. (in: Bacteria)
0.044
-
 NADPH reductive amination at 30°C Bacillus sp. (in: Bacteria)
3.13
-
 2-oxoglutarate reductive amination at 30°C Bacillus sp. (in: Bacteria)
5.96
-
 NH3 reductive amination at 30°C Bacillus sp. (in: Bacteria)
6.06
-
 L-glutamate oxidative deamination at 30°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 alpha6, 6 * 52000, SDS-PAGE Bacillus sp. (in: Bacteria)
315000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Bacillus sp. (in: Bacteria)
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bacillus sp. (in: Bacteria) KSM-635
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) KSM-635
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
416
-
 reductive amination at 30°C Bacillus sp. (in: Bacteria)
1140
-
 reductive amination at pH 7.5 and 60°C Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
-20°C, phosphate buffer at pH 7.0, 0.2 mM 2-mercaptoethanol, 20 h, complete activity loss Bacillus sp. (in: Bacteria)
5°C, phosphate buffer at pH 7.0, 2 mM 2-mercaptoethanol, 6 months, no activity loss Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Bacillus sp. (in: Bacteria) L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Bacillus sp. (in: Bacteria) KSM-635 L-glutamate + H2O + NAD+
-
 r
L-glutamate + NADP+ + H2O
-
 Bacillus sp. (in: Bacteria) 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bacillus sp. (in: Bacteria) KSM-635 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 52000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 reductive amination and oxidative deamination Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 irreversible inactivation Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 reductive amination Bacillus sp. (in: Bacteria)
8.5
-
 oxidative deamination Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Bacillus sp. (in: Bacteria)
NADPH
-
 Bacillus sp. (in: Bacteria)