KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NADPH | reductive amination | Escherichia coli | |
0.043 | - |
NADP+ | oxidative deamination | Psychrobacter sp. | |
0.045 | - |
NADP+ | oxidative deamination | Escherichia coli | |
0.2 | - |
2-oxoglutarate | reductive amination | Escherichia coli | |
2.3 | - |
L-glutamate | oxidative deamination | Escherichia coli | |
2.5 | - |
NH3 | reductive amination | Escherichia coli | |
4 | - |
NH3 | reductive amination | Psychrobacter sp. | |
67.4 | - |
L-glutamate | oxidative deamination | Psychrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
alpha6, 6 * 47000, SDS-PAGE | Psychrobacter sp. |
49285 | - |
alpha6, 6 * 49285, amino acid analysis | Psychrobacter sp. |
290000 | - |
gel filtration | Psychrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Escherichia coli | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Psychrobacter sp. | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Escherichia coli DH5-alpha | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Psychrobacter sp. TAD1 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain DH5alpha | - |
Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Psychrobacter sp. | - |
- |
- |
Psychrobacter sp. TAD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Psychrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78 | - |
reductive amination at 20°C | Psychrobacter sp. |
204.1 | - |
oxidative deamination at 20°C | Psychrobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, 40% v/v glycerol, no activity loss | Psychrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Escherichia coli | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Psychrobacter sp. | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Escherichia coli DH5-alpha | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Psychrobacter sp. TAD1 | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + NADP+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Psychrobacter sp. | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Escherichia coli DH5-alpha | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Psychrobacter sp. TAD1 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 47000, SDS-PAGE | Psychrobacter sp. |
homohexamer | alpha6, 6 * 49285, amino acid analysis | Psychrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Psychrobacter sp. |
55 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
irreversible heat inactivation | Psychrobacter sp. |
61 | - |
50% activity after 5 min | Escherichia coli |
66 | - |
50% activity after 5 min | Psychrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
8 | - |
oxidative deamination at 20°C | Psychrobacter sp. |
9 | - |
- |
Escherichia coli |
9 | - |
reductive amination at 20°C | Psychrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Psychrobacter sp. | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Psychrobacter sp. |