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Literature summary for 1.4.1.3 extracted from

  • Lee, M.; Gonzßlez, J.; Robb, F.
    Extremely thermostable glutamate dehydrogenase (GDH) from the freshwater archaeon Thermococcus waiotapuensis Cloning and comparison with two marine hyperthermophilic GDHs (2002), Extremophiles, 6, 151-159 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermococcus litoralis
-
Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 Thermococcus litoralis
Ca2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 Pyrococcus furiosus
Mg2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 Thermococcus litoralis
Mg2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P80319
-
-
Thermococcus litoralis Q56304
-
-
Thermococcus litoralis DSM 5473 Q56304
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
Thermococcus litoralis 2-oxoglutarate + NH3 + NADPH + H+
-
?
L-glutamate + H2O + NADP+
-
Pyrococcus furiosus 2-oxoglutarate + NH3 + NADPH + H+
-
?
L-glutamate + H2O + NADP+
-
Thermococcus litoralis DSM 5473 2-oxoglutarate + NH3 + NADPH + H+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
97
-
specific activity increases up to 97┬░C Thermococcus litoralis
97
-
specific activity increases up to 97┬░C Pyrococcus furiosus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
104
-
half-life: 4.9 min in absence of KCl, 45 min in presence of 1 M KCl Thermococcus litoralis
104
-
half-life: 67 min in absence of KCl, 562 min in presence of 1 M KCl Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Thermococcus litoralis
8
-
-
Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity Thermococcus litoralis
7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity Pyrococcus furiosus