Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type GLUD1 and GLUD2 in Spodoptera frugiperda Sf21 cells using the baculovirus expression system | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination | Homo sapiens | |
12.8 | - |
NH3 | hGDH1, pH 8.0, temperature not specified in the publication | Homo sapiens | |
14.7 | - |
NH3 | hGDH2, pH 8.0, temperature not specified in the publication | Homo sapiens | |
33 | - |
NH3 | hGDH2, pH 7.5, temperature not specified in the publication | Homo sapiens | |
35 | - |
NH3 | hGDH1, pH 7.5, temperature not specified in the publication | Homo sapiens | |
57.5 | - |
NH3 | hGDH1, pH 7.0, temperature not specified in the publication | Homo sapiens | |
62.2 | - |
NH3 | hGDH2, pH 7.0, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Homo sapiens | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Homo sapiens | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
two human isozymes hGDH1 and hGDH2, genes GLUD1 and GLUD2 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type GLUD1 and GLUD2 from Spodoptera frugiperda Sf21 cells by ammonim sulfate fractionation, and hydrophobic interaction and hydroxyapatite chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
higher activity than at pH 7.0 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | while GDH in most mammals is encoded by a single GLUD1 gene, humans and other primates have acquired a GLUD2 gene with distinct tissue expression profile | Homo sapiens |