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Literature summary for 1.4.1.3 extracted from

  • Amenabar, M.J.; Blamey, J.M.
    Purification and characterization of a thermostable glutamate dehydrogenase from a thermophilic bacterium isolated from a sterilization drying oven (2012), BMB Rep., 45, 91-95.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NADP+ pH 8.0, 70┬░C uncultured bacterium
0.3
-
L-glutamate pH 8.0, 70┬░C uncultured bacterium
0.4
-
NAD+ pH 8.0, 70┬░C uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
6 * 40500, SDS-PAGE uncultured bacterium
250500
-
gel filtration uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ uncultured bacterium
-
2-oxoglutarate + NH3 + NADH + H+
-
ir
L-glutamate + H2O + NADP+ uncultured bacterium
-
2-oxoglutarate + NH3 + NADPH + H+
-
ir

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
GWE1, a Gram-positive microaerophilic microorganism isolated from from the interior of a sterilization drying oven
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 22.7fold by anion exchange chromatography and gel filtration to homogeneity uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
79.4
-
purified enzyme, pH 8.0, 70┬░C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
uncultured bacterium 2-oxoglutarate + NH3 + NADH + H+
-
ir
L-glutamate + H2O + NADP+
-
uncultured bacterium 2-oxoglutarate + NH3 + NADPH + H+
-
ir
additional information no activity in the amination reaction direction with NADH or NADPH, 2-oxoglutarate and ammonia uncultured bacterium ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 40500, SDS-PAGE uncultured bacterium

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
70
-
glutamate oxidation uncultured bacterium

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
50 100 activity range, profile overview. Instability of the coenzyme at temperatures above 95┬░C uncultured bacterium

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
100
-
purified enzyme, 70% activity remaining after over 5 h uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
glutamate oxidation uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
7.9 9 activity range, profile overview uncultured bacterium

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme can use both NAD+ and NADP+ as electron acceptors, displaying more affinity for NADP+ than for NAD+. No activity with NADH or NADPH uncultured bacterium
NAD+
-
uncultured bacterium
NADP+
-
uncultured bacterium