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Literature summary for 1.4.1.3 extracted from

  • Bao, X.; Pal, R.; Hascup, K.N.; Wang, Y.; Wang, W.T.; Xu, W.; Hui, D.; Agbas, A.; Wang, X.; Michaelis, M.L.; Choi, I.Y.; Belousov, A.B.; Gerhardt, G.A.; Michaelis, E.K.
    Transgenic expression of Glud1 (glutamate dehydrogenase 1) in neurons: in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability (2009), J. Neurosci., 29, 13929-13944.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
introducing gene Glud1 under the control of the neuron-specific enolase promoter into mouse brain Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-
synaptosome
-
 Mus musculus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Mus musculus
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+ Mus musculus C57BL/6J x SJL
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NADP+ Mus musculus
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-glutamate + H2O + NADP+ Mus musculus C57BL/6J x SJL
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Mus musculus P26443 super-ovulating C57BL6/SJL hybrid mice, gene GlUD1
-
Mus musculus C57BL/6J x SJL P26443 super-ovulating C57BL6/SJL hybrid mice, gene GlUD1
-

Source Tissue

Source Tissue Comment Organism Textmining
brain GLUD1 expression analysis Mus musculus
-
hippocampus
-
 Mus musculus
-
neuron
-
 Mus musculus
-
striatum
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity in wild-type and transgenic overexpressing brain cell fractions, overview Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Mus musculus 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NAD+
-
 Mus musculus C57BL/6J x SJL 2-oxoglutarate + NH3 + NADH + H+
-
 r
L-glutamate + H2O + NADP+
-
 Mus musculus 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-glutamate + H2O + NADP+
-
 Mus musculus C57BL/6J x SJL 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
GLUD1
-
 Mus musculus
glutamate dehydrogenase 1
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus
NADH
-
 Mus musculus
NADP+
-
 Mus musculus
NADPH
-
 Mus musculus

General Information

General Information Comment Organism
additional information transgenic mice overexpressing the enzyme in brain have decreases in MAP2A labeling of dendrites and in synaptophysin labeling of presynaptic terminals, increasing in neuronal numbers and dendrite and presynaptic terminal labeling with advancing age, and show decreases in long-term potentiation of synaptic activity and in spine density in dendrites of CA1 neurons. Despite overexpression of Glud1 in all neurons of the central nervous system, the Tg mice suffer neuronal losses in select brain regions, e.g., the CA1 but not the CA3 region, dendrite structure and neuronal numbers in brains of transgenic mice, overview. The transgenic mice are significantly more resistant than wild-type mice to induction and duration of anesthesia produced by anesthetics that suppress Glu neurotransmission, phenotype, overview Mus musculus