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Literature summary for 1.4.1.3 extracted from

  • Yoon, H.Y.; Cho, E.H.; Yang, S.J.; Lee, H.J.; Huh, J.W.; Choi, M.M.; Cho, S.W.
    Reactive amino acid residues involved in glutamate-binding of human glutamate dehydrogenase isozymes (2004), Biochimie, 86, 261-267.
    View publication on PubMed
Search for more literature for 1.4.1.3

Activating Compound

Activating Compound Comment Organism Structure
ADP activates Homo sapiens

Protein Variants

Protein Variants Comment Organism
D172Y ratio of turnover number to Km-value for NAD+ is 1.22fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 1.2fold lower than the wild-type value, isoenzyme hGDH1 Homo sapiens
D172Y ratio of turnover number to Km-value for NAD+ is 1.2fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 1.1fold lower than the wild-type value, reduced sensitivity to ADP activation, isoenzyme hGDH2 Homo sapiens
G96Y ratio of turnover number to Km-value for NAD+ is 1.4fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 13.5fold lower than the wild-type value, isoenzyme hGDH1 Homo sapiens
G96Y ratio of turnover number to Km-value for NAD+ is 1.4fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 14.3fold lower than the wild-type value, isoenzyme hGDH2 Homo sapiens
K118Y ratio of turnover number to Km-value for NAD+ is 1.8fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 9.9fold lower than the wild-type value, isoenzyme hGDH1 Homo sapiens
K118Y ratio of turnover number to Km-value for NAD+ is 2fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 8.1fold lower than the wild-type value, isoenzyme hGDH2 Homo sapiens
K130Y ratio of turnover number to Km-value for NAD+ is 26.5fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 35fold lower than the wild-type value, isoenzyme hGDH2 Homo sapiens
K130Y ratio of turnover number to Km-value for NAD+ is 41.6fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 47.3fold lower than the wild-type value, isoenzyme hGDH1 Homo sapiens
K94Y ratio of turnover number to Km-value for NAD+ is 5.6fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 25.5fold lower than the wild-type value, isoenzyme hGDH2 Homo sapiens
K94Y ratio of turnover number to Km-value for NAD+ is 6.6fold lower than the wild-type value. Ratio of turnover number to Km-value for glutamate is 37.8fold lower than the wild-type value, isoenzyme hGDH1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K94Y Homo sapiens
0.58
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2G96Y Homo sapiens
0.59
-
 NAD+ pH 9.5, 25°C, wild-type enzyme hGDH2 Homo sapiens
0.63
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2D172Y Homo sapiens
0.65
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1G96Y Homo sapiens
0.71
-
 NAD+ pH 9.5, 25°C, wild-type enzyme hGDH1 Homo sapiens
0.75
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K130Y Homo sapiens
0.76
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1D172Y Homo sapiens
0.79
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K94Y Homo sapiens
0.82
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K118Y Homo sapiens
0.92
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K130Y Homo sapiens
0.94
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K118Y Homo sapiens
1.77
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2D172Y Homo sapiens
1.81
-
 L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH2 Homo sapiens
2.98
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K130Y Homo sapiens
3.44
-
 L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH1 Homo sapiens
3.71
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1D172Y Homo sapiens
5.51
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K130Y Homo sapiens
7.99
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K94Y Homo sapiens
10.41
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K118Y Homo sapiens
17.75
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2G96Y Homo sapiens
21.82
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K94Y Homo sapiens
25.82
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K118Y Homo sapiens
30.55
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1G96Y Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NAD+ + H2O
-
 Homo sapiens 2-oxoglutarate + NADH + NH3
-
 ?

Synonyms

Synonyms Comment Organism
hGDH1
-
 Homo sapiens
hGDH2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K130Y Homo sapiens
2
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K130Y Homo sapiens
4
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K130Y Homo sapiens
4
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K130Y Homo sapiens
11
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K94Y Homo sapiens
11
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K94Y Homo sapiens
14
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K94Y Homo sapiens
14
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K94Y Homo sapiens
42
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1G96Y Homo sapiens
42
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1G96Y Homo sapiens
48
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1K118Y Homo sapiens
48
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1K118Y Homo sapiens
57
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH1D172Y Homo sapiens
57
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH1D172Y Homo sapiens
57
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2D172Y Homo sapiens
57
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2D172Y Homo sapiens
57
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2G96Y Homo sapiens
57
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2G96Y Homo sapiens
59
-
 NAD+ pH 9.5, 25°C, mutant enzyme hGDH2K118Y Homo sapiens
59
-
 L-glutamate pH 9.5, 25°C, mutant enzyme hGDH2K118Y Homo sapiens
65
-
 NAD+ pH 9.5, 25°C, wild-type enzyme hGDH1 Homo sapiens
65
-
 L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH1 Homo sapiens
83
-
 NAD+ pH 9.5, 25°C, wild-type enzyme hGDH2 Homo sapiens
83
-
 L-glutamate pH 9.5, 25°C, wild-type enzyme hGDH2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens